Scholarlink

Background <p>The bacterial second messenger c-di-GMP is known to influence the formation of biofilms and thereby persistence of pathogenic and beneficial bacteria in hosts. A previous evolution experiment with <i>Pseudomonas lurida</i> MYb11, occasional symbiont of the nematode <i>Caenorhabditis elegans</i>, led to the emergence of host-specialized variants with elevated intracellular c-di-GMP. Thus far, the molecular underpinnings of c-di-GMP-mediated host specialization were unknown in this symbiosis. Therefore, the current study aimed at identifying candidate molecular processes by combining transcriptomic and functional genetic analyses.</p> Results <p>We found that MYb11 host specialists differentially expressed genes related to attachment, motility and biofilm production, including <i>pelD</i> from the <i>pel</i> gene cluster. <i>pelD</i> deletion resulted in reduced intra-host competitive fitness, lower bacterial numbers in <i>C. elegans</i> and loss of biofilm biomass.</p> Conclusion <p>Our results identify <i>pelD</i> as a previously unknown key modulator of beneficial symbiont-host associations that acts downstream of c-di-GMP.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

PelD is required downstream of c-di-GMP for host specialization of Pseudomonas lurida

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月16日

查看全文
  • Anna Czerwinski,
  • Julia Löwenstrom,
  • Sören Franzenburg,
  • Espen Elias Groth,
  • Nancy Obeng,
  • Hinrich Schulenburg

摘要

Background

The bacterial second messenger c-di-GMP is known to influence the formation of biofilms and thereby persistence of pathogenic and beneficial bacteria in hosts. A previous evolution experiment with Pseudomonas lurida MYb11, occasional symbiont of the nematode Caenorhabditis elegans, led to the emergence of host-specialized variants with elevated intracellular c-di-GMP. Thus far, the molecular underpinnings of c-di-GMP-mediated host specialization were unknown in this symbiosis. Therefore, the current study aimed at identifying candidate molecular processes by combining transcriptomic and functional genetic analyses.

Results

We found that MYb11 host specialists differentially expressed genes related to attachment, motility and biofilm production, including pelD from the pel gene cluster. pelD deletion resulted in reduced intra-host competitive fitness, lower bacterial numbers in C. elegans and loss of biofilm biomass.

Conclusion

Our results identify pelD as a previously unknown key modulator of beneficial symbiont-host associations that acts downstream of c-di-GMP.