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Background <p>The emergence of carbapenem resistance in gram-negative bacteria such as <i>Escherichia coli</i> is one of the world’s most urgent public health problems. <i>E. coli</i>, which encounter a diverse range of niches in host can rapidly adapt to the changes in surrounding environment by coordinating their behavior via production, release and detection of signal molecules called autoinducers through a cell density dependent communication system known as quorum sensing. Here, in this study we investigated whether imipenem, and acyl homoserine lactone quorum sensing signal molecules influence the transcriptional response within <i>lsr</i> and <i>lsrRK</i> operon which are associated with auto inducer-2 mediated quorum sensing in <i>E. coli.</i> Two <i>E. coli</i> isolates carrying <i>bla</i><sub>NDM</sub> were treated with 10% SDS for 20 consecutive days, resulting in the successful elimination of the <i>bla</i><sub>NDM</sub> encoding plasmid from one isolate. Plasmid was extracted from the isolate and was transformed into recipient <i>E. coli</i> DH5α by electroporation. The native type, plasmid-cured type, transformant, and <i>E. coli</i> DH5α were allowed to grow under eight different inducing conditions and the transcriptional responses of <i>lsr</i> and <i>lsrRK</i> operons were studied by quantitative real-time PCR method.</p> Results <p>The findings of this study highlight the distinct effects of imipenem and AHL on the transcriptional responses of the <i>lsrB,lsrR</i>, and <i>lsrK</i> genes in native type, plasmid cured type, transformant, and <i>E. coli</i> DH5α.</p> Conclusion <p>This study provides a basis for further research to elucidate different inducing conditions including antibiotics and autoinducers that could switch on the quorum sensing circuit in carbapenem non-susceptible <i>E. coli</i>, one of the world’s most urgent public health threats.</p>

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Transcriptional response study of auto inducer-2 regulatory system in Escherichia coli harboring blaNDM

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月3日

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  • Chandrayee Deshamukhya,
  • Sabnam Ahmed,
  • Bhaskar Jyoti Das,
  • Debadatta Dhar Chanda,
  • Amitabha Bhattacharjee

摘要

Background

The emergence of carbapenem resistance in gram-negative bacteria such as Escherichia coli is one of the world’s most urgent public health problems. E. coli, which encounter a diverse range of niches in host can rapidly adapt to the changes in surrounding environment by coordinating their behavior via production, release and detection of signal molecules called autoinducers through a cell density dependent communication system known as quorum sensing. Here, in this study we investigated whether imipenem, and acyl homoserine lactone quorum sensing signal molecules influence the transcriptional response within lsr and lsrRK operon which are associated with auto inducer-2 mediated quorum sensing in E. coli. Two E. coli isolates carrying blaNDM were treated with 10% SDS for 20 consecutive days, resulting in the successful elimination of the blaNDM encoding plasmid from one isolate. Plasmid was extracted from the isolate and was transformed into recipient E. coli DH5α by electroporation. The native type, plasmid-cured type, transformant, and E. coli DH5α were allowed to grow under eight different inducing conditions and the transcriptional responses of lsr and lsrRK operons were studied by quantitative real-time PCR method.

Results

The findings of this study highlight the distinct effects of imipenem and AHL on the transcriptional responses of the lsrB,lsrR, and lsrK genes in native type, plasmid cured type, transformant, and E. coli DH5α.

Conclusion

This study provides a basis for further research to elucidate different inducing conditions including antibiotics and autoinducers that could switch on the quorum sensing circuit in carbapenem non-susceptible E. coli, one of the world’s most urgent public health threats.