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Background <p>Severe pneumonia in children is a life-threatening respiratory condition that can lead to severe complications and even mortality. We confirmed the possibility of miR-100-5p as a biomarker in severe pneumonia in children.</p> Methods <p>A total of 200 children were included. The expression of miR-100-5p from serum was detected. WI-38 cells were treated with 10&#xa0;µg/mL LPS for 24&#xa0;h. miR-100-5p was overexpressed or inhibited by transfection. In addition, proliferation and inflammatory factors were measured. TargetScan was utilized to forecast the downstream of miR-100-5p. The targeting relationship between miR-100-5p and MTOR was confirmed by the dual-luciferase reporter system. miR-100-5p and MTOR were co-overexpressed to detect the function of both in severe pneumonia in children.</p> Results <p>miR-100-5p expression was decreased and had a high diagnostic value in severe pneumonia in children. The miR-100-5p level was closely related to clinical indicators. In WI-38 cells treated with LPS, overexpression of miR-100-5p increased proliferation and reduced inflammatory factors, while inhibition of miR-100-5p reduced proliferation and increased inflammatory factors. MTOR expression was negatively associated with miR-100-5p expression in severe pneumonia in children.</p> Conclusions <p>miR-100-5p may participate in severe pneumonia in children by negatively regulating MTOR, proving that it might be a biomarker of severe pneumonia in children.</p>

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MiR-100-5p is involved in severe pneumonia in children by targeting MTOR

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月28日

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  • Lun Zhang,
  • Qing Wang,
  • Shoushan Mo

摘要

Background

Severe pneumonia in children is a life-threatening respiratory condition that can lead to severe complications and even mortality. We confirmed the possibility of miR-100-5p as a biomarker in severe pneumonia in children.

Methods

A total of 200 children were included. The expression of miR-100-5p from serum was detected. WI-38 cells were treated with 10 µg/mL LPS for 24 h. miR-100-5p was overexpressed or inhibited by transfection. In addition, proliferation and inflammatory factors were measured. TargetScan was utilized to forecast the downstream of miR-100-5p. The targeting relationship between miR-100-5p and MTOR was confirmed by the dual-luciferase reporter system. miR-100-5p and MTOR were co-overexpressed to detect the function of both in severe pneumonia in children.

Results

miR-100-5p expression was decreased and had a high diagnostic value in severe pneumonia in children. The miR-100-5p level was closely related to clinical indicators. In WI-38 cells treated with LPS, overexpression of miR-100-5p increased proliferation and reduced inflammatory factors, while inhibition of miR-100-5p reduced proliferation and increased inflammatory factors. MTOR expression was negatively associated with miR-100-5p expression in severe pneumonia in children.

Conclusions

miR-100-5p may participate in severe pneumonia in children by negatively regulating MTOR, proving that it might be a biomarker of severe pneumonia in children.