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Background <p>Jiangquan black pig is a novel breed under development, characterized by rapid growth, high-quality meat, strong stress resistance, and high reproductive performance. However, the molecular mechanisms underpinning their muscle development remain poorly characterized. To address this gap, we conducted whole-transcriptome RNA-seq on longissimus dorsi muscle samples from Jiangquan black pigs with divergent growth rates (fast-growing vs. slow-growing) to identify key regulatory factors of muscle development.</p> Results <p>We identified a total of 5,329 known and 331 novel lncRNAs from the transcriptomic data, among which 70 lncRNAs showed differential expression between the fast-growing and slow-growing groups. Functional enrichment analysis of these differentially expressed (DE) lncRNAs revealed that multiple DE-lncRNAs were associated with PPP3CB; three candidate lncRNAs (ENSSSCG00000036609, ENSSSCG00000047932, and the novel lncRNA LOC100518120) were further predicted as potential regulators of muscle growth. Subsequent functional validation in porcine skeletal muscle satellite cells confirmed that LOC100518120 significantly upregulates the expression of its downstream target gene G0S2, promotes cell proliferation and differentiation, and suppresses apoptosis.</p> Conclusions <p>Our study identifies key lncRNA candidates associated with muscle growth in Jiangquan black pig, and elucidates the pro-myogenic functional role of the novel lncRNA LOC100518120. These findings provide a theoretical foundation for optimizing molecular breeding strategies and further improving meat quality in this breed.</p>

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Identification of differentially expressed lncRNAs in different daily weight gains of Jiangquan black pigs and functional analysis of LOC100518120

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月6日

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  • Jingsen Huang,
  • Hongzhen Cao,
  • Xinlin Jin,
  • Qi Song,
  • Wei Chen,
  • Hui Tang,
  • Yunzhou Wang,
  • Yongqing Zeng

摘要

Background

Jiangquan black pig is a novel breed under development, characterized by rapid growth, high-quality meat, strong stress resistance, and high reproductive performance. However, the molecular mechanisms underpinning their muscle development remain poorly characterized. To address this gap, we conducted whole-transcriptome RNA-seq on longissimus dorsi muscle samples from Jiangquan black pigs with divergent growth rates (fast-growing vs. slow-growing) to identify key regulatory factors of muscle development.

Results

We identified a total of 5,329 known and 331 novel lncRNAs from the transcriptomic data, among which 70 lncRNAs showed differential expression between the fast-growing and slow-growing groups. Functional enrichment analysis of these differentially expressed (DE) lncRNAs revealed that multiple DE-lncRNAs were associated with PPP3CB; three candidate lncRNAs (ENSSSCG00000036609, ENSSSCG00000047932, and the novel lncRNA LOC100518120) were further predicted as potential regulators of muscle growth. Subsequent functional validation in porcine skeletal muscle satellite cells confirmed that LOC100518120 significantly upregulates the expression of its downstream target gene G0S2, promotes cell proliferation and differentiation, and suppresses apoptosis.

Conclusions

Our study identifies key lncRNA candidates associated with muscle growth in Jiangquan black pig, and elucidates the pro-myogenic functional role of the novel lncRNA LOC100518120. These findings provide a theoretical foundation for optimizing molecular breeding strategies and further improving meat quality in this breed.