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<p>The increasing emergence of multidrug-resistant (MDR) <i>Klebsiella pneumoniae</i> strains has become a threat to public health, and it is important to understand the mechanisms underlying adaptive evolution. In this study, we characterized a clinical MDR isolate of <i>K. pneumoniae</i>, designated KPA1, which belongs to sequence type 15 (ST15), along with its two associated resistance plasmids, pKPA1-1 and pKPA1-2. Conjugation assays demonstrated that both plasmids were capable of self-transfer. Notably, pKPA1-1 could be transferred to both <i>K. pneumoniae</i> and <i>Escherichia coli</i>, while pKPA1-2 exhibited a restricted transfer capability, being transferable only to <i>K. pneumoniae</i>. Antimicrobial susceptibility testing, along with plasmid stability assays, indicated that the resistance spectrum of pKPA1-2 similar to that of pKPA1-1, and pKPA1-2 was more stable than pKPA1-1. In addition, virulence assays revealed that the acquisition of either plasmid resulted in a reduction in bacterial pathogenicity. This study elucidates the molecular characterization of conjugative resistance plasmids pKPA1-1 and pKPA1-2. These findings shed light on the adaptive mechanisms underlying multidrug resistance in <i>K. pneumoniae</i>.</p>

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Molecular characterization of two conjugative resistance plasmids from a clinical multidrug-resistant ST15 Klebsiella pneumoniae

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月16日

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  • Ruixue Tao,
  • Meng Li,
  • Nan Zhang,
  • Linke Cao,
  • Jie Zhang,
  • Hanqi Li,
  • Wenbo Yan,
  • Yiming Yang,
  • Tietao Wang,
  • Yani Zhang,
  • Ke Xing

摘要

The increasing emergence of multidrug-resistant (MDR) Klebsiella pneumoniae strains has become a threat to public health, and it is important to understand the mechanisms underlying adaptive evolution. In this study, we characterized a clinical MDR isolate of K. pneumoniae, designated KPA1, which belongs to sequence type 15 (ST15), along with its two associated resistance plasmids, pKPA1-1 and pKPA1-2. Conjugation assays demonstrated that both plasmids were capable of self-transfer. Notably, pKPA1-1 could be transferred to both K. pneumoniae and Escherichia coli, while pKPA1-2 exhibited a restricted transfer capability, being transferable only to K. pneumoniae. Antimicrobial susceptibility testing, along with plasmid stability assays, indicated that the resistance spectrum of pKPA1-2 similar to that of pKPA1-1, and pKPA1-2 was more stable than pKPA1-1. In addition, virulence assays revealed that the acquisition of either plasmid resulted in a reduction in bacterial pathogenicity. This study elucidates the molecular characterization of conjugative resistance plasmids pKPA1-1 and pKPA1-2. These findings shed light on the adaptive mechanisms underlying multidrug resistance in K. pneumoniae.