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Background <p>Long noncoding RNAs (lncRNAs) have been shown to play important roles in plant abiotic stress response and adaptation. However, the identification and characterization of genome-wide drought-responsive lncRNAs in rapeseed (<i>Brassica napus</i> L.) have been limited. Therefore, this study was the first to identify the expression profile of lncRNAs in rapeseed seeds responding to prolonged drought stress and subsequent short-term rewatering.</p> Results <p>A total of 6 000 lncRNAs were identified, among which 181 were classified as differentially expressed lncRNAs (DELs) in response to either drought stress or subsequent rewatering. Comparative analysis revealed that only 14 DELs were shared between the 159 DELs identified during drought stress and the 27 DELs detected upon rewatering. GO enrichment analysis showed that the co-expressed DEGs, primarily involved in photosynthesis, central carbon metabolism, stomatal movement, and strigolactone metabolism, were significantly down-regulated under drought stress but markedly up-regulated in subsequent rewatering. Furthermore, drought‑responsive competing endogenous RNA (ceRNA) networks were constructed based on the identified DETs and DEGs. Two ceRNA modules, MSTRG.57345.1-bna-miR164a/b/c/d-HSP2 and MSTRG.57345.1-bna-miR395d/e/f-HSFA7a, which are based on the newly identified lncRNA MSTRG.57345.1, were detected for the first time in rapeseed under drought stress and subsequent rewatering.</p> Conclusions <p>The present study advances our understanding of the expression patterns and functional role of rapeseed lncRNAs in the response to drought stress and the subsequent rewatering. It provides novel insights into lncRNA-mRNA networks and lncRNA-miRNA-mRNA networks in the seeds of <i>B. napus</i>. These findings offer a valuable reference for further genetic research and molecular breeding programs aimed at rapeseed improvement.</p>

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Genome-wide analysis of long noncoding RNAs in response to drought stress in the seeds of Brassica napus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月6日

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  • Kai Zhang,
  • Wenbing Wang,
  • Junrong Li,
  • Zhen Liang,
  • Maoteng Li

摘要

Background

Long noncoding RNAs (lncRNAs) have been shown to play important roles in plant abiotic stress response and adaptation. However, the identification and characterization of genome-wide drought-responsive lncRNAs in rapeseed (Brassica napus L.) have been limited. Therefore, this study was the first to identify the expression profile of lncRNAs in rapeseed seeds responding to prolonged drought stress and subsequent short-term rewatering.

Results

A total of 6 000 lncRNAs were identified, among which 181 were classified as differentially expressed lncRNAs (DELs) in response to either drought stress or subsequent rewatering. Comparative analysis revealed that only 14 DELs were shared between the 159 DELs identified during drought stress and the 27 DELs detected upon rewatering. GO enrichment analysis showed that the co-expressed DEGs, primarily involved in photosynthesis, central carbon metabolism, stomatal movement, and strigolactone metabolism, were significantly down-regulated under drought stress but markedly up-regulated in subsequent rewatering. Furthermore, drought‑responsive competing endogenous RNA (ceRNA) networks were constructed based on the identified DETs and DEGs. Two ceRNA modules, MSTRG.57345.1-bna-miR164a/b/c/d-HSP2 and MSTRG.57345.1-bna-miR395d/e/f-HSFA7a, which are based on the newly identified lncRNA MSTRG.57345.1, were detected for the first time in rapeseed under drought stress and subsequent rewatering.

Conclusions

The present study advances our understanding of the expression patterns and functional role of rapeseed lncRNAs in the response to drought stress and the subsequent rewatering. It provides novel insights into lncRNA-mRNA networks and lncRNA-miRNA-mRNA networks in the seeds of B. napus. These findings offer a valuable reference for further genetic research and molecular breeding programs aimed at rapeseed improvement.