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Background <p><i>Maclura tricuspidata</i> is an important medicinal and horticultural plant. However, the complete mitochondrial genome (mitogenome) of <i>M. tricuspidata</i> has not been reported, hindering molecular phylogenetic studies, species identification, and evolutionary research.</p> Results <p>We present the first comprehensive analysis of the <i>M. tricuspidata</i> mitogenome. It features a multi-chromosomal structure comprising three circular-mapping chromosomes, with a total length of 416,801&#xa0;bp and a GC content of 44.94%. Annotation identified 28 unique protein-coding genes (PCGs), 18 tRNA genes, and 3 rRNA genes. Codon usage analysis revealed GCU and CAA as the predominant codons for alanine and glutamine, respectively, while methionine and tryptophan, as single-codon amino acids, showed no bias. A total of 154 simple sequence repeats (SSRs) were detected: 84 on chromosome 1, 48 on chromosome 2, and 22 on chromosome 3. Analysis identified 19 homologous fragments transferred from the chloroplast genome (cpDNA), accounting for 4.31% of the mitogenome length. Using the Deepred-mt suite, 409&#xa0;C-to-U RNA editing sites were predicted from the complete set of 28 mitochondrial PCGs, with the highest number in <i>nad4</i> and the lowest in <i>sdh4</i>. Phylogenetic analysis confirmed the placement of <i>M. tricuspidata</i> within the Moraceae family, showing closest relationships to <i>Ficus carica</i> and <i>Morus notabilis</i>, consistent with the APG IV system. Comparative analysis revealed extensive syntenic blocks between the <i>M. tricuspidata</i> mitogenome and those of related species, alongside regions lacking homology. In addition, dN/dS analysis revealed that most of the protein-coding genes in the mitogenome had undergone negative selection, and only the <i>ccmB</i> and <i>sdh4</i> gene had undergone potential positive selection in <i>M. tricuspidata</i>.</p> Conclusions <p>The unique structural features and complexities of the <i>M. tricuspidata</i> mitogenome, along with its similarities and differences compared to related species, provide valuable insights into plant mitochondrial evolution, energy metabolism, and environmental adaptation. These findings contribute significantly to the understanding of plant mitogenome diversity and biology.</p>

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Assembly and comparative analysis of the complete mitochondrial genome of the Maclura tricuspidata

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月5日

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  • Shengkui Zhang,
  • Xia Wang,
  • Xianyan Zhao,
  • Ziyang Gao,
  • Kun Pan

摘要

Background

Maclura tricuspidata is an important medicinal and horticultural plant. However, the complete mitochondrial genome (mitogenome) of M. tricuspidata has not been reported, hindering molecular phylogenetic studies, species identification, and evolutionary research.

Results

We present the first comprehensive analysis of the M. tricuspidata mitogenome. It features a multi-chromosomal structure comprising three circular-mapping chromosomes, with a total length of 416,801 bp and a GC content of 44.94%. Annotation identified 28 unique protein-coding genes (PCGs), 18 tRNA genes, and 3 rRNA genes. Codon usage analysis revealed GCU and CAA as the predominant codons for alanine and glutamine, respectively, while methionine and tryptophan, as single-codon amino acids, showed no bias. A total of 154 simple sequence repeats (SSRs) were detected: 84 on chromosome 1, 48 on chromosome 2, and 22 on chromosome 3. Analysis identified 19 homologous fragments transferred from the chloroplast genome (cpDNA), accounting for 4.31% of the mitogenome length. Using the Deepred-mt suite, 409 C-to-U RNA editing sites were predicted from the complete set of 28 mitochondrial PCGs, with the highest number in nad4 and the lowest in sdh4. Phylogenetic analysis confirmed the placement of M. tricuspidata within the Moraceae family, showing closest relationships to Ficus carica and Morus notabilis, consistent with the APG IV system. Comparative analysis revealed extensive syntenic blocks between the M. tricuspidata mitogenome and those of related species, alongside regions lacking homology. In addition, dN/dS analysis revealed that most of the protein-coding genes in the mitogenome had undergone negative selection, and only the ccmB and sdh4 gene had undergone potential positive selection in M. tricuspidata.

Conclusions

The unique structural features and complexities of the M. tricuspidata mitogenome, along with its similarities and differences compared to related species, provide valuable insights into plant mitochondrial evolution, energy metabolism, and environmental adaptation. These findings contribute significantly to the understanding of plant mitogenome diversity and biology.