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<p>Cucumber (<i>Cucumis sativus</i>), a key vegetable crop, is highly sensitive to water deficiency. MicroRNAs (miRNAs ) are small non-coding RNAs (18–25 nucleotides), which regulate gene expression via sequence-specific binding, and are crucial for plant growth, development, and stress responses. To explore drought-responsive miRNAs and their targets in <i>C. sativus</i>, transcriptome, miRNA, and degradome sequencing were conducted on drought-stressed cucumber leaves. A total of 7,871 differentially expressed mRNAs and 57 differentially expressed miRNAs (55 known and 2 novel) were identified. Degradome analysis revealed 43 miRNAs targeting 129 genes, forming 164 miRNA-target gene pairs, which were primarily involved in secondary metabolite biosynthesis and abiotic stress responses. Among them, 11 key miRNA-target gene pairs linked to drought response were highlighted. This study offered valuable molecular insights into miRNA-mediated drought regulation in cucumbers, thereby contributing our understanding of their stress adaptation mechanisms.</p>

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Integrated analyses reveal drought responsive microRNAs and their target genes in cucumber

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月5日

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  • Yi Xiao,
  • Lili Guan,
  • Zhaolong Sun,
  • Yifei Du,
  • Danni Xu,
  • Keling Hu,
  • Defang Gan

摘要

Cucumber (Cucumis sativus), a key vegetable crop, is highly sensitive to water deficiency. MicroRNAs (miRNAs ) are small non-coding RNAs (18–25 nucleotides), which regulate gene expression via sequence-specific binding, and are crucial for plant growth, development, and stress responses. To explore drought-responsive miRNAs and their targets in C. sativus, transcriptome, miRNA, and degradome sequencing were conducted on drought-stressed cucumber leaves. A total of 7,871 differentially expressed mRNAs and 57 differentially expressed miRNAs (55 known and 2 novel) were identified. Degradome analysis revealed 43 miRNAs targeting 129 genes, forming 164 miRNA-target gene pairs, which were primarily involved in secondary metabolite biosynthesis and abiotic stress responses. Among them, 11 key miRNA-target gene pairs linked to drought response were highlighted. This study offered valuable molecular insights into miRNA-mediated drought regulation in cucumbers, thereby contributing our understanding of their stress adaptation mechanisms.