Scholarlink

Background <p>Herbivorous teleosts represent eco-efficient alternatives for sustainable aquaculture, yet the molecular drivers of herbivory in hybrid culter remain elusive. We investigated the hybrid <i>culter</i> (BTBT)—derived from <i>Megalobrama amblycephala</i> (blunt snout bream, BSB, ♀) and <i>Culter alburnus</i> (topmouth culter, TC, ♂)—exhibiting transitional herbivory.</p> Results <p>Morphometric analyses revealed BTBT pharyngeal dentition (formula 2.4.5–5.4.2) and intestinal coiling (IL/BL = 1.63) intermediate between parental phenotypes. After a 12-week 30% protein dietary intervention, digestive enzyme assays demonstrated BTBT’s gut cellulase activity (76.02 U/g) mirrored herbivorous BSB (79.83 U/g; <i>P</i> &gt; 0.05) and exceeded carnivorous TC (47.71 U/g; <i>P</i> &lt; 0.05). The 16S rRNA profiling identified enrichment of cellulolytic <i>Flavobacterium</i> and elevated carbohydrate-metabolizing pathways in BTBT. Liver transcriptomics further revealed superior xenobiotic detoxification and glycolytic plasticity in herbivorous BTBT. Multi-omics correlation networks exposed synergistic host-microbiota interactions governing plant polysaccharide utilization and enteric homeostasis. Crucially, <i>h6pd</i> emerged as a nodal regulator, orchestrating NADPH-dependent glycolipid equilibrium, free radical scavenging, and xenobiotic metabolism.</p> Conclusions <p>Our findings establish that herbivory in BTBT arises through tripartite adaptation: Digestive system structural adaptation, symbiotic microbiota-driven enzymology, and conserved regulatory genetics.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Multi-omics interrogation of herbivorous phenotype acquisition in hybrid culter under a 30% crude protein diet

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月19日

查看全文
  • Yuxiang Wang,
  • Jinhui Huang,
  • Ming Wen,
  • Hongxuan Liang,
  • Yingying Yang,
  • Lang Qin,
  • Le Shen,
  • Jiawang Huang,
  • Xu Huang,
  • Zhuangwen Mao,
  • Fangzhou Hu,
  • Chang Wu,
  • Shaojun Liu

摘要

Background

Herbivorous teleosts represent eco-efficient alternatives for sustainable aquaculture, yet the molecular drivers of herbivory in hybrid culter remain elusive. We investigated the hybrid culter (BTBT)—derived from Megalobrama amblycephala (blunt snout bream, BSB, ♀) and Culter alburnus (topmouth culter, TC, ♂)—exhibiting transitional herbivory.

Results

Morphometric analyses revealed BTBT pharyngeal dentition (formula 2.4.5–5.4.2) and intestinal coiling (IL/BL = 1.63) intermediate between parental phenotypes. After a 12-week 30% protein dietary intervention, digestive enzyme assays demonstrated BTBT’s gut cellulase activity (76.02 U/g) mirrored herbivorous BSB (79.83 U/g; P > 0.05) and exceeded carnivorous TC (47.71 U/g; P < 0.05). The 16S rRNA profiling identified enrichment of cellulolytic Flavobacterium and elevated carbohydrate-metabolizing pathways in BTBT. Liver transcriptomics further revealed superior xenobiotic detoxification and glycolytic plasticity in herbivorous BTBT. Multi-omics correlation networks exposed synergistic host-microbiota interactions governing plant polysaccharide utilization and enteric homeostasis. Crucially, h6pd emerged as a nodal regulator, orchestrating NADPH-dependent glycolipid equilibrium, free radical scavenging, and xenobiotic metabolism.

Conclusions

Our findings establish that herbivory in BTBT arises through tripartite adaptation: Digestive system structural adaptation, symbiotic microbiota-driven enzymology, and conserved regulatory genetics.