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Background <p>Lyme borreliosis (LB), the most common vector-borne disease in the Northern Hemisphere, is caused by a multitude of pathogenic <i>Borrelia burgdorferi</i> sensu lato (sl) species endemic to northern, western, and central regions of Europe. We utilized whole genome sequencing (WGS) to characterize the genetic diversity of 130 clinical <i>B. burgdorferi</i> sl isolates collected from LB cases in the Netherlands between 1988 and 2023, the majority of which were <i>B. afzelii</i>.</p> Results <p>WGS analysis revealed significant diversity, including 29 different multi-locus sequence types (MLSTs) across four genospecies. Plasmids from <i>B. garinii</i> and <i>B. bavariensis</i> were found to exhibit greater sequence diversity than those from <i>B. burgdorferi</i> sensu stricto and <i>B. afzelii</i>. We further characterized the <i>Borrelia</i> membrane protein antigens OspA, OspC and DbpA for sequence diversity and correlation with LB disease state.</p> Conclusions <p>This large-scale genomic analysis of clinical Dutch <i>B. burgdorferi</i> sl isolates furthers our understanding of LB and may indicate potential coverage for LB vaccine candidates in clinical development.</p>

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Genomic characterization of clinical Borrelia burgdorferi sensu lato isolates in the Netherlands over a thirty-year period

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月27日

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  • Zhenghui Li,
  • Jonathan T. Lee,
  • Varun Raghuraman,
  • Lorna D. Nunez,
  • Urvi Rajyaguru,
  • Amber Vrijlandt,
  • Katrina E. Llamera,
  • Lubomira Andrew,
  • Alje P. Van Dam,
  • Annaliesa S. Anderson,
  • Paul A. Liberator,
  • Li Hao,
  • Raphael Simon,
  • Joppe W. Hovius

摘要

Background

Lyme borreliosis (LB), the most common vector-borne disease in the Northern Hemisphere, is caused by a multitude of pathogenic Borrelia burgdorferi sensu lato (sl) species endemic to northern, western, and central regions of Europe. We utilized whole genome sequencing (WGS) to characterize the genetic diversity of 130 clinical B. burgdorferi sl isolates collected from LB cases in the Netherlands between 1988 and 2023, the majority of which were B. afzelii.

Results

WGS analysis revealed significant diversity, including 29 different multi-locus sequence types (MLSTs) across four genospecies. Plasmids from B. garinii and B. bavariensis were found to exhibit greater sequence diversity than those from B. burgdorferi sensu stricto and B. afzelii. We further characterized the Borrelia membrane protein antigens OspA, OspC and DbpA for sequence diversity and correlation with LB disease state.

Conclusions

This large-scale genomic analysis of clinical Dutch B. burgdorferi sl isolates furthers our understanding of LB and may indicate potential coverage for LB vaccine candidates in clinical development.