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Background <p><i>Lagerstroemia speciosa</i> ‘Zijuan’ is a new variety of <i>L. speciosa</i> with highly petaloid stamens that exhibit ornamental value but sterility. To investigate the mechanism of stamen petaloidy, this study compared the morphological structures and transcriptomic profiles of stamens between <i>L. speciosa</i> and <i>L. speciosa</i> ‘Zijuan’.</p> Results <p>Stamen petaloidy in <i>L. speciosa</i> ‘Zijuan’ initiates when bud diameters reached approximately 3&#xa0;mm. During three developmental stages—stamen primordium (buds 1–2&#xa0;mm), anther formation (buds 2–3&#xa0;mm), and anther development (buds 3–4&#xa0;mm)—8122, 8058, and 7242 differentially expressed genes (DEGs) were identified between the two varieties, respectively. GO enrichment analysis revealed that most DEGs related to tapetum development and photoperiod sensing in <i>L. speciosa</i> ‘Zijuan’ were upregulated during stamen development compared to <i>L. speciosa</i>. KEGG pathway analysis identified activated auxin, gibberellins, and jasmonic acid response factors in hormone signaling pathways. Expression differences in A-, B-, and C-class genes were observed across the three stages: A- and B-class genes showed minimal changes, while C-class gene expression was suppressed. Weighted gene co-expression network analysis (WGCNA) analysis of C-class co-expression networks suggested potential associations between <i>AGAMOUS</i> and its homolog <i>MADS3</i> with <i>SMD1B</i>. Marked expression disparities in these genes were confirmed between <i>L. speciosa</i> and <i>L. speciosa</i> ‘Zijuan’ flower buds. RT-qPCR results corroborated transcriptome data, validating reliability.</p> Conclusions <p>This study proposes novel regulatory mechanisms for stamen petaloidy, providing technical insights for future investigations into stamen development, petaloidy mechanisms, and candidate gene selection in <i>L. speciosa</i>.</p>

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Comparative transcriptomic analysis of petaloid stamen formation in Lagerstroemia speciosa ‘Zijuan’

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月19日

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  • Baojin Zhang,
  • Ruoyong Yin,
  • Shuaijie Lu,
  • Lan Wang,
  • Fei Xiao,
  • Ruchun Xi,
  • Xiaomei Deng

摘要

Background

Lagerstroemia speciosa ‘Zijuan’ is a new variety of L. speciosa with highly petaloid stamens that exhibit ornamental value but sterility. To investigate the mechanism of stamen petaloidy, this study compared the morphological structures and transcriptomic profiles of stamens between L. speciosa and L. speciosa ‘Zijuan’.

Results

Stamen petaloidy in L. speciosa ‘Zijuan’ initiates when bud diameters reached approximately 3 mm. During three developmental stages—stamen primordium (buds 1–2 mm), anther formation (buds 2–3 mm), and anther development (buds 3–4 mm)—8122, 8058, and 7242 differentially expressed genes (DEGs) were identified between the two varieties, respectively. GO enrichment analysis revealed that most DEGs related to tapetum development and photoperiod sensing in L. speciosa ‘Zijuan’ were upregulated during stamen development compared to L. speciosa. KEGG pathway analysis identified activated auxin, gibberellins, and jasmonic acid response factors in hormone signaling pathways. Expression differences in A-, B-, and C-class genes were observed across the three stages: A- and B-class genes showed minimal changes, while C-class gene expression was suppressed. Weighted gene co-expression network analysis (WGCNA) analysis of C-class co-expression networks suggested potential associations between AGAMOUS and its homolog MADS3 with SMD1B. Marked expression disparities in these genes were confirmed between L. speciosa and L. speciosa ‘Zijuan’ flower buds. RT-qPCR results corroborated transcriptome data, validating reliability.

Conclusions

This study proposes novel regulatory mechanisms for stamen petaloidy, providing technical insights for future investigations into stamen development, petaloidy mechanisms, and candidate gene selection in L. speciosa.