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Background <p>Blueberry (<i>Vaccinium spp.</i>) celebrated for its rich nutritional content and significant health benefits, was referred to as the ‘Queen of Fruits’ and the ‘King of Berries’. The development of blueberry fruits is closely related to plant hormones. Glycosylation mediated by UDP-glycosyltransferases (UGTs) is a key step in plant hormones homeostasis. However, the UGT members has not been reported in blueberry to date.</p> Results <p>A total of 361 <i>VcUGT</i> genes were identified and classified into 11 groups. Whole-genome and segmental duplications drove <i>VcUGT</i> expansion, with structural analysis revealing conservation within subgroups but divergence among them. Group G members were associated with cytokinin glycosylation, particularly dihydrozeatin, which promotes fruit enlargement. RT-qPCR suggested <i>VcUGTs</i> involvement in fruit development, while functional validation confirmed VcUGT160 localization to the nucleus and cell membrane, likely mediating dihydrozeatin glycosylation.</p> Conclusions <p>The results of this study identified and characterized the UGT gene family in Blueberry. VcUGT160 may function in mediating dihydrozeatin glycosylation. Moreover, these findings enhance our understanding of VcUGTs evolution and function in blueberry.</p>

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Genome-wide characterization of the UDP-glycosyltransferases (UGT) family and functional analysis of VcUGT160 involved in dihydrozeatin glycosylation during blueberry fruits development

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月14日

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  • Yifei Wang,
  • Xinlei Liu,
  • Tong Zhao,
  • Jing Li,
  • Li Chen,
  • Yadong Li,
  • Haiyue Sun

摘要

Background

Blueberry (Vaccinium spp.) celebrated for its rich nutritional content and significant health benefits, was referred to as the ‘Queen of Fruits’ and the ‘King of Berries’. The development of blueberry fruits is closely related to plant hormones. Glycosylation mediated by UDP-glycosyltransferases (UGTs) is a key step in plant hormones homeostasis. However, the UGT members has not been reported in blueberry to date.

Results

A total of 361 VcUGT genes were identified and classified into 11 groups. Whole-genome and segmental duplications drove VcUGT expansion, with structural analysis revealing conservation within subgroups but divergence among them. Group G members were associated with cytokinin glycosylation, particularly dihydrozeatin, which promotes fruit enlargement. RT-qPCR suggested VcUGTs involvement in fruit development, while functional validation confirmed VcUGT160 localization to the nucleus and cell membrane, likely mediating dihydrozeatin glycosylation.

Conclusions

The results of this study identified and characterized the UGT gene family in Blueberry. VcUGT160 may function in mediating dihydrozeatin glycosylation. Moreover, these findings enhance our understanding of VcUGTs evolution and function in blueberry.