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Background <p>Phosphorus (P) is an essential nutrient for plant growth and development. Phosphate transporter 1 (PHT1) is a transmembrane protein that mediates the uptake and translocation of inorganic phosphate (Pi) in plants. Despite extensive research on the PHT1 family across various species, the <i>PHT1</i> genes in <i>Eucalyptus grandis</i> are poorly documented and not identified comprehensively.</p> Results <p>In this study, a total of 21 <i>EgrPHT1</i>genes (<i>EgrPHT1-1</i> ~ <i>EgrPHT1-21</i>) were identified for the comprehensive analysis of the whole genome of <i>E</i>. <i>grandis</i>. Gene structure showed that the number of exons ranges from 1 to 6, and 57% gene members lacking introns. Protein multi-sequence alignment analysis revealed that all members except the <i>EgrPHT1-5</i> had 12 transmembrane domains. Chromosome localization analysis showed that all <i>EgrPHT1</i> genes were unevenly distributed on 8 chromosomes. Collinearity result identified one pair of tandem duplication and two pairs of fragment duplications. Promoter <i>cis</i>-elements suggested that the expression of the <i>EgrPHT1</i> genes was affected by various hormonal and abiotic stresses. Tissue-specific expression patterns indicated six genes (<i>EgrPHT1-9</i>/<i>10</i>/<i>13</i>/<i>15</i>/<i>16</i>/<i>17</i>) is specifically expressed in the roots, indicating that they may be involved in the formation and development of the root. Furthermore, 9 <i>EgrPHT1</i> gene members (<i>EgrPHT1-1/3/4/10/11/12/15/16/19</i>) were upregulated in the root and <i>EgrPHT1-1</i>/<i>3</i>/<i>4</i>/<i>1</i>/<i>16</i>/<i>19</i> was also upregulated in leaves under phosphorus deficiency.</p> Conclusions <p>In this study, we identified and analyzed EgrPHT1 family genes using bioinformatics approaches, and also examined differential expression patterns of <i>EgrPHT1</i> genes in various tissues and in response to Pi starvation stress, suggesting their involvement in P uptake, translocation, and homeostasis. This information provides a platform for further investigation of their biological functions.</p>

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Identification of the phosphate transporter 1 family genes in the Eucalyptus grandis genome and their expression under different phosphate regimes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月8日

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  • Juan Li,
  • Yanli Xu,
  • Jiaru Liu,
  • Jiayue Liu,
  • Zhaohua Lu,
  • Jianmin Xu,
  • Guangyou Li

摘要

Background

Phosphorus (P) is an essential nutrient for plant growth and development. Phosphate transporter 1 (PHT1) is a transmembrane protein that mediates the uptake and translocation of inorganic phosphate (Pi) in plants. Despite extensive research on the PHT1 family across various species, the PHT1 genes in Eucalyptus grandis are poorly documented and not identified comprehensively.

Results

In this study, a total of 21 EgrPHT1genes (EgrPHT1-1 ~ EgrPHT1-21) were identified for the comprehensive analysis of the whole genome of E. grandis. Gene structure showed that the number of exons ranges from 1 to 6, and 57% gene members lacking introns. Protein multi-sequence alignment analysis revealed that all members except the EgrPHT1-5 had 12 transmembrane domains. Chromosome localization analysis showed that all EgrPHT1 genes were unevenly distributed on 8 chromosomes. Collinearity result identified one pair of tandem duplication and two pairs of fragment duplications. Promoter cis-elements suggested that the expression of the EgrPHT1 genes was affected by various hormonal and abiotic stresses. Tissue-specific expression patterns indicated six genes (EgrPHT1-9/10/13/15/16/17) is specifically expressed in the roots, indicating that they may be involved in the formation and development of the root. Furthermore, 9 EgrPHT1 gene members (EgrPHT1-1/3/4/10/11/12/15/16/19) were upregulated in the root and EgrPHT1-1/3/4/1/16/19 was also upregulated in leaves under phosphorus deficiency.

Conclusions

In this study, we identified and analyzed EgrPHT1 family genes using bioinformatics approaches, and also examined differential expression patterns of EgrPHT1 genes in various tissues and in response to Pi starvation stress, suggesting their involvement in P uptake, translocation, and homeostasis. This information provides a platform for further investigation of their biological functions.