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<p><i>Mussaenda anomala</i> exhibits high sensitivity to low-temperature stress, but its molecular adaptation mechanisms remain poorly understood. In this study, tissue-cultured seedlings were exposed to temperature gradients (25&#xa0;°C as control, 12&#xa0;°C, 4&#xa0;°C, and 0&#xa0;°C) to investigate cold-stress responses. Physiological analyses revealed increased oxidative damage (increased levels of H₂O₂, relative electrolyte conductivity (REC) and malondialdehyde (MDA)) and chloroplast/mitochondrial impairment, accompanied by downregulation of photosynthetic genes (Psb/Psa/LHCA/LHCB). The plants activated multi-level defenses, including stomatal closure, palisade tissue thickening, starch accumulation, and upregulation of starch/sucrose metabolism genes (SS/Amy). Antioxidant systems (SOD/POD/CAT) were enhanced, alongside hormonal reprogramming (ABA/JA accumulation with auxin suppression). Weighted gene co-expression network analysis (WGCNA) revealed three co-expression modules containing 12 hub genes (BKI1-like, PAO2-like, CDPK1 etc.) potentially regulating cold adaptation. These findings provide the first comprehensive molecular characterization of <i>M. anomala</i>’s cold stress response, offering valuable targets for breeding cold-tolerant ornamental plants.</p>

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Integrated physiological, biochemical, and transcriptomic analysis of the cold-sensitive response in Mussaenda anomala

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月10日

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  • Zhongcheng Peng,
  • Yingliang Liu,
  • Xuan Tan,
  • Zhongwei He,
  • Wulan Huang,
  • Dayi Xie,
  • Shiyi Mu,
  • Qian Huang,
  • Yunjie Hu,
  • Lili Zhan,
  • Ping Gui,
  • Xiao Wang,
  • Guanglin Tao,
  • Yang Yang,
  • Gaoyin Wu

摘要

Mussaenda anomala exhibits high sensitivity to low-temperature stress, but its molecular adaptation mechanisms remain poorly understood. In this study, tissue-cultured seedlings were exposed to temperature gradients (25 °C as control, 12 °C, 4 °C, and 0 °C) to investigate cold-stress responses. Physiological analyses revealed increased oxidative damage (increased levels of H₂O₂, relative electrolyte conductivity (REC) and malondialdehyde (MDA)) and chloroplast/mitochondrial impairment, accompanied by downregulation of photosynthetic genes (Psb/Psa/LHCA/LHCB). The plants activated multi-level defenses, including stomatal closure, palisade tissue thickening, starch accumulation, and upregulation of starch/sucrose metabolism genes (SS/Amy). Antioxidant systems (SOD/POD/CAT) were enhanced, alongside hormonal reprogramming (ABA/JA accumulation with auxin suppression). Weighted gene co-expression network analysis (WGCNA) revealed three co-expression modules containing 12 hub genes (BKI1-like, PAO2-like, CDPK1 etc.) potentially regulating cold adaptation. These findings provide the first comprehensive molecular characterization of M. anomala’s cold stress response, offering valuable targets for breeding cold-tolerant ornamental plants.