Scholarlink

Background <p>The unique feeding behavior of <i>Siniperca chuatsi</i> (<i>S. chuatsi</i>), which exclusively consumes live fry and rejects dead fry, severely limits the development of its aquaculture industry. This rejection of dead fry likely stems from an aversion to necromones (death-associated odors). Although a subset of trace amine-associated receptors (TAARs) containing key conserved residues (Asp<sup>3.32</sup> and/or Asp<sup>5.42</sup>) are implicated in detecting amine-related odors, the <i>taar</i> gene members in <i>S. chuatsi</i> and their responsiveness to necromones remain unexplored.</p> Results <p>In this study, we found that the aversion of <i>S. chuatsi</i> to dead prey is associated with necromones. Furthermore, we identified 68 <i>taar</i> family members in the <i>S. chuatsi</i> genome, distributed across six chromosomes, with 36 tandem duplication events generating 54 duplicated genes. Phylogenetic analysis classified <i>taar</i>s into two classes, Class I and Class III, with a total of eight subfamilies. Synteny analysis revealed low synteny between the <i>taar</i>s of <i>S. chuatsi</i> and <i>Dicentrarchus labrax</i>, with only 12 pairs of genes showing syntenic relationships. Protein sequence analysis demonstrated that <i>taar</i>s contain amine recognition sites, <i>N</i>-glycosylation, and conserved cysteine structures, with high conservation and purifying selection observed within subfamilies. Subcellular localization revealed that TAARs are localized to the cell membrane and nucleus. Transcriptome data indicated that 61 <i>taar</i>s are expressed in the olfactory epithelium, while only 19 are expressed in the brain. The response of representative taars to putrescine and cadaverine was divergent when comparing olfactory and brain tissues.</p> Conclusions <p>This study systematically identified the <i>taar</i> gene family in <i>S. chuatsi</i>, analyzing their evolutionary relationships, protein structures, subcellular localization, expression profiles in olfactory and brain tissues, and the response of selected TAARs to necromones (cadaverine and putrescine). This work establishes a foundation for further investigation into TAAR-mediated death odor recognition in <i>S. chuatsi</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide identification, expression analysis of taars (trace amine-associated receptors) and response to cadaverine and putrescine in Mandarin fish (Siniperca chuatsi)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月27日

查看全文
  • Xiaoru Dong,
  • Maolin Lv,
  • Qiyao Luo,
  • Xiao Jiang,
  • Xu-fang Liang

摘要

Background

The unique feeding behavior of Siniperca chuatsi (S. chuatsi), which exclusively consumes live fry and rejects dead fry, severely limits the development of its aquaculture industry. This rejection of dead fry likely stems from an aversion to necromones (death-associated odors). Although a subset of trace amine-associated receptors (TAARs) containing key conserved residues (Asp3.32 and/or Asp5.42) are implicated in detecting amine-related odors, the taar gene members in S. chuatsi and their responsiveness to necromones remain unexplored.

Results

In this study, we found that the aversion of S. chuatsi to dead prey is associated with necromones. Furthermore, we identified 68 taar family members in the S. chuatsi genome, distributed across six chromosomes, with 36 tandem duplication events generating 54 duplicated genes. Phylogenetic analysis classified taars into two classes, Class I and Class III, with a total of eight subfamilies. Synteny analysis revealed low synteny between the taars of S. chuatsi and Dicentrarchus labrax, with only 12 pairs of genes showing syntenic relationships. Protein sequence analysis demonstrated that taars contain amine recognition sites, N-glycosylation, and conserved cysteine structures, with high conservation and purifying selection observed within subfamilies. Subcellular localization revealed that TAARs are localized to the cell membrane and nucleus. Transcriptome data indicated that 61 taars are expressed in the olfactory epithelium, while only 19 are expressed in the brain. The response of representative taars to putrescine and cadaverine was divergent when comparing olfactory and brain tissues.

Conclusions

This study systematically identified the taar gene family in S. chuatsi, analyzing their evolutionary relationships, protein structures, subcellular localization, expression profiles in olfactory and brain tissues, and the response of selected TAARs to necromones (cadaverine and putrescine). This work establishes a foundation for further investigation into TAAR-mediated death odor recognition in S. chuatsi.