Scholarlink

Background <p>Enhancing flag leaf nitrogen use efficiency (NUE) in wheat production can substantially increase crop productivity while minimizing nitrogen application. Quantitative trait loci (QTLs) for NUE-related have been rarely reported in wheat flag leaf traits.</p> Results <p>In this study, a natural population of 243 varieties and an RIL population of 123 F<sub>7</sub> recombinants were subjected to different nitrogen treatments. A genome-wide association study (GWAS) and linkage analysis were performed for four agronomic traits in terms of flag leaf length, flag leaf width, flag leaf area, and SPAD (chlorophyll content) under low and normal nitrogen conditions. Through GWAS, 1,016 significant SNP loci were identified and clustered into 290 QTLs, including 11 stably mapped QTLs (stable detection in multiple environments). Additionally, an AC population was established to verify the GWAS results and identify reliable QTL intervals. Three stable loci, namely, <i>QFLLR6D.3 QFLWR6A.6</i>, and <i>QSPADR5B.3</i>, were validated in the AC population, located 1.34&#xa0;Mb, 2.84&#xa0;Mb, and 5&#xa0;Mb away from linkage mapping significant QTL, respectively. Through further transcriptome analysis of Chilero leaves at the jointing, anthesis and grain filling stages, four DEGs were identified within <i>QSPADR5B.3</i>. Among them, <i>TraesCS5B02G394300</i>, <i>TraesCS5B02G394200</i>, and <i>TraesCS5B02G39390</i> encode beta-glucosidases, and <i>TraesCS5B02G396400</i> encodes a potassium channel.</p> Conclusions <p>These findings offer potential candidate genes for wheat breeding, and provide a foundation for exploring the molecular targets underlying wheat NUE.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide association study, linkage mapping and transcriptomic analysis revealed candidate genes with the flag leaf traits associated with nitrogen use efficiency in wheat

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月26日

查看全文
  • Yulin Jia,
  • Ninglu Xu,
  • Jinzhi Wu,
  • Chunping Wang,
  • Ming Huang,
  • Youjun Li

摘要

Background

Enhancing flag leaf nitrogen use efficiency (NUE) in wheat production can substantially increase crop productivity while minimizing nitrogen application. Quantitative trait loci (QTLs) for NUE-related have been rarely reported in wheat flag leaf traits.

Results

In this study, a natural population of 243 varieties and an RIL population of 123 F7 recombinants were subjected to different nitrogen treatments. A genome-wide association study (GWAS) and linkage analysis were performed for four agronomic traits in terms of flag leaf length, flag leaf width, flag leaf area, and SPAD (chlorophyll content) under low and normal nitrogen conditions. Through GWAS, 1,016 significant SNP loci were identified and clustered into 290 QTLs, including 11 stably mapped QTLs (stable detection in multiple environments). Additionally, an AC population was established to verify the GWAS results and identify reliable QTL intervals. Three stable loci, namely, QFLLR6D.3 QFLWR6A.6, and QSPADR5B.3, were validated in the AC population, located 1.34 Mb, 2.84 Mb, and 5 Mb away from linkage mapping significant QTL, respectively. Through further transcriptome analysis of Chilero leaves at the jointing, anthesis and grain filling stages, four DEGs were identified within QSPADR5B.3. Among them, TraesCS5B02G394300, TraesCS5B02G394200, and TraesCS5B02G39390 encode beta-glucosidases, and TraesCS5B02G396400 encodes a potassium channel.

Conclusions

These findings offer potential candidate genes for wheat breeding, and provide a foundation for exploring the molecular targets underlying wheat NUE.