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<p>The<!--Query ID="Q1" Text="Please check if the article title is captured correctly." Resolved="yes"--> ectoparasite <i>Varroa destructor</i> is the primary global threat to the western honey bee, <i>Apis mellifera</i>. Growing resistance to acaricide-based treatments has spurred interest in alternative control strategies. In this study, we employed a novel and efficient dsRNA delivery method to explore the <!--Query ID="Q2" Text="Please check if the affiliations are presented correctly." Resolved="yes"-->potential of RNA interference (RNAi)-based approaches for <i>Varroa</i> control in honey bee colonies. We assessed the effects of silencing six target genes (<i>ptch1</i>, <i>ap-1</i>, <i>larp6</i>, <i>chisal</i>, <i>vg1</i>, and <i>vg6</i>) on mite mortality and reproduction through a semi-field experiment. Gene expression analysis revealed significantly reduced transcript levels in mites treated with dsRNA compared to dsGFP controls, with knockdown efficiencies ranging from 88.6% to 97.2%. Silencing of <i>ptch1</i>, <i>ap-1</i>, and <i>vg1</i> genes resulted in a significant increase in mite infertility,<!--Query ID="Q3" Text="Please confirm if the author name is presented accurately." Resolved="yes"--> aligning with their known roles in oocyte maturation and embryogenesis. Additionally, silencing of <i>chisal</i>, previously described as essential for effective <i>Varroa</i> feeding, led to a marked increase in mite mortality. These results highlight promising gene targets for RNAi-based <i>Varroa</i> control strategies. Furthermore, our study provides new insights into the molecular pathways involved in mite reproduction and survival, including Wnt, c-Jun N-terminal kinase, Hedgehog, and apoptosis, paving the way for the development of more effective biotechnological control tools.</p>

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Assessing the role of key genes involved in the reproductive success of the honey bee parasite Varroa destructor

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月1日

查看全文
  • Irina Muntaabski,
  • Ricardo Salvador,
  • Romina M. Russo,
  • Juan P. Wulff,
  • Lucas Landi,
  • María C. Liendo,
  • Silvia B. Lanzavecchia,
  • Alejandra C. Scannapieco

摘要

The ectoparasite Varroa destructor is the primary global threat to the western honey bee, Apis mellifera. Growing resistance to acaricide-based treatments has spurred interest in alternative control strategies. In this study, we employed a novel and efficient dsRNA delivery method to explore the potential of RNA interference (RNAi)-based approaches for Varroa control in honey bee colonies. We assessed the effects of silencing six target genes (ptch1, ap-1, larp6, chisal, vg1, and vg6) on mite mortality and reproduction through a semi-field experiment. Gene expression analysis revealed significantly reduced transcript levels in mites treated with dsRNA compared to dsGFP controls, with knockdown efficiencies ranging from 88.6% to 97.2%. Silencing of ptch1, ap-1, and vg1 genes resulted in a significant increase in mite infertility, aligning with their known roles in oocyte maturation and embryogenesis. Additionally, silencing of chisal, previously described as essential for effective Varroa feeding, led to a marked increase in mite mortality. These results highlight promising gene targets for RNAi-based Varroa control strategies. Furthermore, our study provides new insights into the molecular pathways involved in mite reproduction and survival, including Wnt, c-Jun N-terminal kinase, Hedgehog, and apoptosis, paving the way for the development of more effective biotechnological control tools.