Background The 14–3-3 proteins are highly conserved regulatory eukaryotic proteins, which are crucial in growth, development, and stress responses. However, systematic characterization of the 14–3-3 gene family in Brassicaceae species and their evolutionary relationships have not been comprehensively reported. Results This study conducted genome-wide identification, structural characteristics, and comparative evolutionary analysis of 14–3-3 gene family members in Arabidopsis thaliana, A. lyrata, A. pumila, Camelina sativa, and Brassica oleracea using comparative genomics. Overall, a total of 108 14–3-3 genes, which were phylogenetically classified into ε and non-ε groups were identified in the five species, with the non-ε members exhibiting more similar exon–intron structures and conserved motif patterns. Collinearity analysis revealed that the Brassicaceae 14–3-3 gene family members underwent varying degrees of expansion following whole-genome duplication (WGD) events. Notably, the number of 14–3-3 gene family members between A. lyrata and A. thaliana remained similar despite the former having approximately 1.66-fold larger genome size. In contrast, the number of 14–3-3 gene family members in A. pumila and C. sativa increased in proportionately to their genome size, while gene members in the more distantly related species to A. thaliana, B. oleracea, showed irregular expansion patterns. Selection pressure analysis revealed that 14–3-3 homologs in all the five species underwent purifying selection, with the group ε members experiencing relatively weaker purifying selection. Cloning of ApGRF6-2 gene from A. pumila indicated that the ApGRF6-2 protein was localized in the cell membrane and cytoplasm, while ectopic overexpression of ApGRF6-2 in A. thaliana could promote early flowering by upregulating the expression of floral meristem identity genes. Conclusion This study provides a comprehensive and systematic identification of the 14–3-3 gene family members in five Brassicaceae species using updated genome sequences, and the results could form a basis for further validation of functional and molecular mechanisms of 14–3-3 genes in plant growth, development, abiotic stress responses, as well as flowering regulation.

Genome-wide identification and comparative evolution of 14–3-3 gene family members in five Brassicaceae species

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2025年3月29日

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Jingya Zhao,
Shengqin Liu,
Hui Ren,
Owusu Edwin Afriyie,
Mengzhu Zhang,
Dachao Xu,
Xianzhong Huang

摘要

Background

The 14–3-3 proteins are highly conserved regulatory eukaryotic proteins, which are crucial in growth, development, and stress responses. However, systematic characterization of the 14–3-3 gene family in Brassicaceae species and their evolutionary relationships have not been comprehensively reported.

Results

This study conducted genome-wide identification, structural characteristics, and comparative evolutionary analysis of 14–3-3 gene family members in Arabidopsis thaliana, A. lyrata, A. pumila, Camelina sativa, and Brassica oleracea using comparative genomics. Overall, a total of 108 14–3-3 genes, which were phylogenetically classified into ε and non-ε groups were identified in the five species, with the non-ε members exhibiting more similar exon–intron structures and conserved motif patterns. Collinearity analysis revealed that the Brassicaceae 14–3-3 gene family members underwent varying degrees of expansion following whole-genome duplication (WGD) events. Notably, the number of 14–3-3 gene family members between A. lyrata and A. thaliana remained similar despite the former having approximately 1.66-fold larger genome size. In contrast, the number of 14–3-3 gene family members in A. pumila and C. sativa increased in proportionately to their genome size, while gene members in the more distantly related species to A. thaliana, B. oleracea, showed irregular expansion patterns. Selection pressure analysis revealed that 14–3-3 homologs in all the five species underwent purifying selection, with the group ε members experiencing relatively weaker purifying selection. Cloning of ApGRF6-2 gene from A. pumila indicated that the ApGRF6-2 protein was localized in the cell membrane and cytoplasm, while ectopic overexpression of ApGRF6-2 in A. thaliana could promote early flowering by upregulating the expression of floral meristem identity genes.

Conclusion

This study provides a comprehensive and systematic identification of the 14–3-3 gene family members in five Brassicaceae species using updated genome sequences, and the results could form a basis for further validation of functional and molecular mechanisms of 14–3-3 genes in plant growth, development, abiotic stress responses, as well as flowering regulation.