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Objective <p>The genus <i>Streptococcus</i> constitutes a major component of the human oral microbiome and exhibits substantial genomic diversity at the species level. However, complete and high-quality reference genomes remain limited for many closely related oral streptococci, constraining accurate comparative and taxonomic analyses. The objective of this Data Note was to generate and report a complete genome sequence of <i>Streptococcus</i> sp. strain KHUD_013, isolated from the human buccal mucosa, and to provide a genomic context for comparison with closely related species.</p> Data description <p>Long-read sequencing using the PacBio Revio platform generated 89,500 high-fidelity (HiFi) reads with a mean read length of 6,960&#xa0;bp. Genome assembly produced a single circular chromosome of 1,988,919&#xa0;bp with a G + C content of 42.5%. Genome annotation identified 1,827 protein-coding genes, four ribosomal RNA operons (5&#xa0;S, 16&#xa0;S, and 23&#xa0;S), and 62 transfer RNA genes, with an estimated completeness of 98.8%. Average Nucleotide Identity (ANI) analysis indicated that strain KHUD_013 is most closely related to the <i>Streptococcus ilei</i> type strain (GCA_000479335.1), with a value of 94.39% and an alignment coverage of 86.55%, consistent with genomic divergence at the species boundary. Comparative genomic analysis with four publicly available <i>S. ilei</i> genomes identified 179 gene clusters unique to KHUD_013, predominantly associated with replication, recombination, and repair, as well as cell wall and membrane biogenesis and transcription. This genome sequence provides a valuable resource for strain-level comparative and taxonomic studies of oral streptococci.</p>

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Complete genome sequence of Streptococcus sp. strain KHUD_013 isolated from the human buccal mucosa

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月28日

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  • Doyun Ku,
  • Seok Bin Yang,
  • Kyu Hwan Kwack,
  • Eun-Young Jang,
  • Soo-Kyung Jun,
  • Ji-Hoi Moon,
  • Jae-Hyung Lee

摘要

Objective

The genus Streptococcus constitutes a major component of the human oral microbiome and exhibits substantial genomic diversity at the species level. However, complete and high-quality reference genomes remain limited for many closely related oral streptococci, constraining accurate comparative and taxonomic analyses. The objective of this Data Note was to generate and report a complete genome sequence of Streptococcus sp. strain KHUD_013, isolated from the human buccal mucosa, and to provide a genomic context for comparison with closely related species.

Data description

Long-read sequencing using the PacBio Revio platform generated 89,500 high-fidelity (HiFi) reads with a mean read length of 6,960 bp. Genome assembly produced a single circular chromosome of 1,988,919 bp with a G + C content of 42.5%. Genome annotation identified 1,827 protein-coding genes, four ribosomal RNA operons (5 S, 16 S, and 23 S), and 62 transfer RNA genes, with an estimated completeness of 98.8%. Average Nucleotide Identity (ANI) analysis indicated that strain KHUD_013 is most closely related to the Streptococcus ilei type strain (GCA_000479335.1), with a value of 94.39% and an alignment coverage of 86.55%, consistent with genomic divergence at the species boundary. Comparative genomic analysis with four publicly available S. ilei genomes identified 179 gene clusters unique to KHUD_013, predominantly associated with replication, recombination, and repair, as well as cell wall and membrane biogenesis and transcription. This genome sequence provides a valuable resource for strain-level comparative and taxonomic studies of oral streptococci.