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Objective <p><i>Streptococcus hominis</i> is a recently described species within the genus <i>Streptococcus</i>, yet its genomic characteristics remain poorly understood, particularly in the context of the oral microbiome. Previously, only two complete genomes from non-oral sources were available. To address this gap, we sequenced and analyzed <i>S. hominis</i> strain KHUD_010, isolated from the subgingival biofilm of a healthy Korean adult.</p> Data description <p>Genomic DNA from KHUD_010 was extracted and confirmed as <i>S. hominis</i> by 16&#xa0;S rRNA gene sequencing. Whole-genome sequencing using the PacBio Sequel II platform generated 135,974 HiFi reads (N50: 10,345&#xa0;bp). <i>De novo</i> assembly with SMRT Link v11.0 produced a single circular chromosome of 1,883,665&#xa0;bp with 39.04% GC content. Annotation <i>via</i> the NCBI Prokaryotic Genome Annotation Pipeline predicted 1,793 protein-coding genes, four rRNA operons (5&#xa0;S, 16&#xa0;S, 23&#xa0;S), and 120 tRNAs. BUSCO analysis showed 99.1% completeness. Comparative genomics with NSJ-17 and UMB6992B revealed 1,416 core, 223 dispensable, and 398 strain-specific gene clusters. KHUD_010 harbored 18 unique gene clusters comprising 20 genes, mostly assigned to COG category L (replication, recombination, repair). This high-quality genome expands the genomic landscape of <i>S. hominis</i> and provides a valuable reference for future studies on oral microbiome diversity and host adaptation.</p>

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Complete genome sequence of Streptococcus hominis isolated from subgingival biofilm

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月29日

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  • Seok Bin Yang,
  • Doyun Ku,
  • Ji-Hoi Moon,
  • Jae-Hyung Lee,
  • Sang Wook Kang,
  • Hak Kyun Kim,
  • Kyu Hwan Kwack

摘要

Objective

Streptococcus hominis is a recently described species within the genus Streptococcus, yet its genomic characteristics remain poorly understood, particularly in the context of the oral microbiome. Previously, only two complete genomes from non-oral sources were available. To address this gap, we sequenced and analyzed S. hominis strain KHUD_010, isolated from the subgingival biofilm of a healthy Korean adult.

Data description

Genomic DNA from KHUD_010 was extracted and confirmed as S. hominis by 16 S rRNA gene sequencing. Whole-genome sequencing using the PacBio Sequel II platform generated 135,974 HiFi reads (N50: 10,345 bp). De novo assembly with SMRT Link v11.0 produced a single circular chromosome of 1,883,665 bp with 39.04% GC content. Annotation via the NCBI Prokaryotic Genome Annotation Pipeline predicted 1,793 protein-coding genes, four rRNA operons (5 S, 16 S, 23 S), and 120 tRNAs. BUSCO analysis showed 99.1% completeness. Comparative genomics with NSJ-17 and UMB6992B revealed 1,416 core, 223 dispensable, and 398 strain-specific gene clusters. KHUD_010 harbored 18 unique gene clusters comprising 20 genes, mostly assigned to COG category L (replication, recombination, repair). This high-quality genome expands the genomic landscape of S. hominis and provides a valuable reference for future studies on oral microbiome diversity and host adaptation.