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Objective <p>This study presents the whole-genome sequence of <i>Staphylococcus aureus</i> strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate’s resistance and virulence potential.</p> Data description <p>: Genomic DNA of <i>S. aureus</i> strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980&#xa0;bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.</p>

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Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月12日

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  • Danladi Makeri,
  • Emmanuel Eilu,
  • Martin Odoki,
  • Ismail Abiola Adebayo,
  • Reuben Maghembe,
  • Musoba Abubakar,
  • Reagan Muhwezi,
  • Theophilus Pius,
  • Priscilla Peter Dilli,
  • Saheed Adekunle Akinola,
  • Ezera Agwu

摘要

Objective

This study presents the whole-genome sequence of Staphylococcus aureus strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate’s resistance and virulence potential.

Data description

: Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.