Background <p>Primary cilia are essential sensory organelles, and their dysfunction leads to a broad spectrum of diseases known as ciliopathies. Immunofluorescence imaging of ciliary substructures is a key tool in cilia research, but multiplex staining is often limited by the availability of antibodies from different host species.</p> Methods <p>We developed a novel goat-derived gamma tubulin antibody for basal body labelling and validated its specificity through triple-label immunofluorescence in different cell lines.</p> Results <p>The antibody showed strong, specific staining of basal bodies under methanol fixation, enabling clear visualization in combination with rabbit- and mouse-derived antibodies.</p> Conclusions <p>This antibody expands multiplex imaging capabilities for ciliary substructures, supporting both cilia research and diagnostics of ciliopathies.</p>