Scholarlink

Background <p>The differentiation of mesodermal cells (MCs) in the early stage of embryonic development contributes to the organogenesis of several core organs. However, the single-cell molecular architecture of MCs and the key molecular events during the differentiation remain unclear.</p> Methods <p>We performed single-cell RNA sequencing (RNA sequencing) and single-cell assay for transposase-accessible chromatin (ATAC-Seq) to analyze the developmental features of MCs to heart, kidney, spleen, liver, and brain in human embryos at gestational ages 7–17 weeks.</p> Results <p>We found that <i>EGR1</i> might be relevant to the differentiation of heterogeneous MC sub-clusters. Meanwhile, <i>RPL10P9</i><sup><i>+</i></sup><i>PTMAP5</i><sup><i>+</i></sup> MCs had the closest expression profiling with endocardial cells. <i>NDUFA4L2</i><sup><i>+</i></sup><i>A2M</i><sup><i>+</i></sup> MCs presented the potentials to form endothelial cells (ECs) and hematopoietic stem cells, and <i>MEF2C</i> might be involved in this process.</p> Conclusions <p>These findings provide insights into the molecular architecture and lineage progression of MCs during early human embryonic organogenesis, offering a valuable reference for regenerative medicine and organ bioengineering.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular architecture of mesoderm cells across early to middle stage of human embryo development at single-cell resolution

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月25日

查看全文
  • Wei Zhang,
  • Haiyan Yu,
  • Zhibin Zhang,
  • Dandan Li,
  • Yane Yang,
  • Wei Shi,
  • Wenting Li,
  • Yan Jiang,
  • Wenlong Hu,
  • Zhipeng Zeng,
  • Xinqiong Liu,
  • Zhanye Zheng,
  • Minglin Ou,
  • Donge Tang,
  • Yong Dai

摘要

Background

The differentiation of mesodermal cells (MCs) in the early stage of embryonic development contributes to the organogenesis of several core organs. However, the single-cell molecular architecture of MCs and the key molecular events during the differentiation remain unclear.

Methods

We performed single-cell RNA sequencing (RNA sequencing) and single-cell assay for transposase-accessible chromatin (ATAC-Seq) to analyze the developmental features of MCs to heart, kidney, spleen, liver, and brain in human embryos at gestational ages 7–17 weeks.

Results

We found that EGR1 might be relevant to the differentiation of heterogeneous MC sub-clusters. Meanwhile, RPL10P9+PTMAP5+ MCs had the closest expression profiling with endocardial cells. NDUFA4L2+A2M+ MCs presented the potentials to form endothelial cells (ECs) and hematopoietic stem cells, and MEF2C might be involved in this process.

Conclusions

These findings provide insights into the molecular architecture and lineage progression of MCs during early human embryonic organogenesis, offering a valuable reference for regenerative medicine and organ bioengineering.