Background <p>Kompetitive Allele-Specific PCR (KASP) is a fluorescence-based, high-throughput and cost-effective genotyping technology widely used for detecting single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels) across various species. However, few software tools are available for automatically designing KASP primers, especially for InDel variations.</p> Results <p>To address the lack of free and user-friendly automated tools for KASP primer design, we analyzed the sequence characteristics of KASP primers and developed a user-friendly program named EasyKASP on the Excel VBA platform. EasyKASP designs KASP primers for both SNP and InDel variations, with an average processing time of only 0.03&#xa0;s per primer pair. A total of 80 SNP loci and 6 InDel loci with variations of different lengths were selected to validate the KASP markers designed by EasyKASP, all of which were successfully amplified and genotyped using KASP technology.</p> Conclusions <p>EasyKASP is a simple and rapid tool for KASP primer design, demonstrating broad applicability in KASP genotyping studies.</p>

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EasyKASP: a simple and fast tool for KASP primer design

  • Jian Zhang,
  • Jingjing Yang,
  • Changlong Wen

摘要

Background

Kompetitive Allele-Specific PCR (KASP) is a fluorescence-based, high-throughput and cost-effective genotyping technology widely used for detecting single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels) across various species. However, few software tools are available for automatically designing KASP primers, especially for InDel variations.

Results

To address the lack of free and user-friendly automated tools for KASP primer design, we analyzed the sequence characteristics of KASP primers and developed a user-friendly program named EasyKASP on the Excel VBA platform. EasyKASP designs KASP primers for both SNP and InDel variations, with an average processing time of only 0.03 s per primer pair. A total of 80 SNP loci and 6 InDel loci with variations of different lengths were selected to validate the KASP markers designed by EasyKASP, all of which were successfully amplified and genotyped using KASP technology.

Conclusions

EasyKASP is a simple and rapid tool for KASP primer design, demonstrating broad applicability in KASP genotyping studies.