Scholarlink

Objective <p>The targets and active components that affect the prognosis of cervical cancer remain unclear.</p> Methods <p>Three microarray datasets were collected for differentially expressed genes (DEGs) screening. The pathway, function, protein interaction and prognosis were analysed. The active components, related targets, the active ingredient-target gene network were conducted. The protein expression of the hub gene was validated through immunohistochemistry and Nanodrugs Que-Fe NDs were prepared and validated by in vitro cellular functionalization experiment.</p> Results <p>162 upregulated and 145 downregulated DEGs were screened out, including 10 hub genes, which were mainly clustered in DNA replication, cell division, proliferation, and cycle. The results of active ingredient-target gene network showed that the traditional Chinese medicine quercetin has the potential therapeutic effect on cervical cancer via targeting PCNA. Experiments focused on cellular functionalization demonstrated that the synthesized Que-Fe nanodots (NDs) were capable of decreasing the levels of proliferating cell nuclear antigen (PCNA) expression and suppressing the in vitro proliferation of HeLa cells.</p> Conclusion <p>The growth of cervical cancer HeLa cells was suppressed in vitro by Que-Fe NDs. This finding indicates a promising novel therapeutic target that warrants additional investigation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Quercetin-Fe nanodots inhibit cervical cancer cell proliferation by targeting PCNA

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月3日

查看全文
  • Youwei Zhou,
  • Sheng Cheng,
  • Lei Shu,
  • Wenzhu He,
  • Ying Chen,
  • Yan Wang,
  • Xuqing Li,
  • Zhaolian Wei

摘要

Objective

The targets and active components that affect the prognosis of cervical cancer remain unclear.

Methods

Three microarray datasets were collected for differentially expressed genes (DEGs) screening. The pathway, function, protein interaction and prognosis were analysed. The active components, related targets, the active ingredient-target gene network were conducted. The protein expression of the hub gene was validated through immunohistochemistry and Nanodrugs Que-Fe NDs were prepared and validated by in vitro cellular functionalization experiment.

Results

162 upregulated and 145 downregulated DEGs were screened out, including 10 hub genes, which were mainly clustered in DNA replication, cell division, proliferation, and cycle. The results of active ingredient-target gene network showed that the traditional Chinese medicine quercetin has the potential therapeutic effect on cervical cancer via targeting PCNA. Experiments focused on cellular functionalization demonstrated that the synthesized Que-Fe nanodots (NDs) were capable of decreasing the levels of proliferating cell nuclear antigen (PCNA) expression and suppressing the in vitro proliferation of HeLa cells.

Conclusion

The growth of cervical cancer HeLa cells was suppressed in vitro by Que-Fe NDs. This finding indicates a promising novel therapeutic target that warrants additional investigation.