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<p>Accumulation of xenobiotic chlorinated ethenes (CEs) at legacy industrial soil and groundwater sites around the world is a pressing environmental and public health issue. Understanding the biochemical pathways through which microorganisms degrade <i>c</i>DCE is key to developing cost-effective, sustainable bioremediation strategies for CE contamination. Two strains, <i>Acinetobacter pittii</i> CEP14 and <i>Ectopseudomonas alcaliphila</i> JAB1, isolated from contaminated industrial sites, have demonstrated the ability to cometabolically degrade <i>c</i>DCE in the presence of phenol. In this study, we integrate transcriptomics, using differential gene expression analysis to pinpoint genes induced during <i>c</i>DCE co‐metabolism, with proteomics to confirm protein‐level expression. We use heterologous expression experiments to demonstrate that phenol monooxygenase is responsible for oxidising <i>c</i>DCE in both strains. Furthermore, we show that CEP14 and JAB1 α-subunits share 71.4% identity with each other but only 14.6–26.5% identity with established monooxygenases with known <i>c</i>DCE-oxidising activity, highlighting the diversity of enzymes that may be capable of cometabolic <i>c</i>DCE degradation. Finally, we hypothesise on a two-branch phenol monooxygenase-mediated <i>c</i>DCE degradation pathway in which the chemical degradative intermediates 2,2-dichloroacetaldehyde and <i>c</i>DCE epoxides are formed. This study sheds light on the biochemical mechanisms by which monoaromatic compounds can enhance the biodegradation of <i>c</i>DCE and demonstrates the potential utilisation of strains CEP14 and JAB1 for the biodegradation of <i>c</i>DCE.</p>

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Phenol-driven cometabolic degradation of cis-1,2-dichloroethene (cDCE): insights from Acinetobacter pittii and Ectopseudomonas alcaliphila

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月7日

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  • Miguel Desmarais,
  • Serena Fraraccio,
  • Jakub Ridl,
  • Jachym Suman,
  • Andre Perez-Potti,
  • Kenneth A. Dawson,
  • Iva Dolinova,
  • Lenka McGachy,
  • Miluse Hradilova,
  • Alena Sevcu,
  • Michal Strejcek,
  • Ondrej Uhlik

摘要

Accumulation of xenobiotic chlorinated ethenes (CEs) at legacy industrial soil and groundwater sites around the world is a pressing environmental and public health issue. Understanding the biochemical pathways through which microorganisms degrade cDCE is key to developing cost-effective, sustainable bioremediation strategies for CE contamination. Two strains, Acinetobacter pittii CEP14 and Ectopseudomonas alcaliphila JAB1, isolated from contaminated industrial sites, have demonstrated the ability to cometabolically degrade cDCE in the presence of phenol. In this study, we integrate transcriptomics, using differential gene expression analysis to pinpoint genes induced during cDCE co‐metabolism, with proteomics to confirm protein‐level expression. We use heterologous expression experiments to demonstrate that phenol monooxygenase is responsible for oxidising cDCE in both strains. Furthermore, we show that CEP14 and JAB1 α-subunits share 71.4% identity with each other but only 14.6–26.5% identity with established monooxygenases with known cDCE-oxidising activity, highlighting the diversity of enzymes that may be capable of cometabolic cDCE degradation. Finally, we hypothesise on a two-branch phenol monooxygenase-mediated cDCE degradation pathway in which the chemical degradative intermediates 2,2-dichloroacetaldehyde and cDCE epoxides are formed. This study sheds light on the biochemical mechanisms by which monoaromatic compounds can enhance the biodegradation of cDCE and demonstrates the potential utilisation of strains CEP14 and JAB1 for the biodegradation of cDCE.