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Objective <p>Understanding age-related changes in migraine is pivotal, considering the increasing global life expectancy. In addition, both aging and migraine are prominent cardiovascular risk factors. It remains unclear whether calcitonin gene-related peptide (CGRP) release changes with age across trigeminovascular components, and how this relates to peripheral responses to migraine-related vasodilatory molecules. The primary aim was to investigate age-related effects on CGRP release from the trigeminovascular system by studying a mouse model of combined accelerated neuronal and vascular aging, the DNA repair-deficient <i>Ercc1</i><sup><i>Δ/−</i></sup> mice. Second, we assessed the effects of aging on isolated coronary vasodilatory responses to CGRP and forskolin.</p> Methods <p>Experiments were conducted using DNA repair-deficient <i>Ercc1</i><sup><i>Δ/−</i></sup>mice and their wild type controls. After sacrifice, the trigeminal nucleus caudalis (TNC), trigeminal ganglion (TG), and dura mater (DM) were isolated. Ex vivo KCl-induced CGRP release was measured, and CGRP release was compared between <i>Ercc1</i><sup><i>Δ/−</i></sup> and wild type mice. In a subset of mice, concentration-response curves to CGRP and forskolin were generated in isolated coronary arteries. The pEC<sub>50</sub> (negative log of the molar concentration of an agonist needed to reach half of its maximal effect) and E<sub>max</sub> (maximum relaxation response) values were compared between both groups.</p> Results <p>CGRP release (expressed as ratio compared to baseline release) of the DM was significantly lower in <i>Ercc1</i><sup><i>Δ/−</i></sup> (2.01 ± 0.24) <i>versus</i> wild type mice (3.22 ± 0.48) (P = 0.040). No differences were observed in CGRP release between <i>Ercc1</i><sup><i>Δ/−</i></sup>and wild type mice in the TNC (8.07 ± 1.15 <i>versus</i> 6.10 ± 0.57, P = 0.364) or the TG (4.84 ± 0.92 <i>versus</i> 4.41 ± 0.60, P = 0.838). In addition, there were no differences in pEC<sub>50</sub> and E<sub>max</sub> values in response to CGRP and forskolin.</p> Conclusion <p>Our findings suggest that aging is linked to reduced CGRP release of the DM, potentially partly explaining the reduction in migraine attacks in elderly. This decreased CGRP release is not accompanied by altered peripheral vascular reactivity to CGRP.</p>

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Trigeminovascular calcitonin gene-related peptide release and peripheral vascular responses in a mouse model of accelerated aging: Implications for migraine

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月3日

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  • Linda Al-Hassany,
  • Eloisa Rubio-Beltrán,
  • Alejandro Labastida-Ramirez,
  • Ingrid M. Garrelds,
  • A. H. Jan Danser,
  • Anton J. M. Roks,
  • Antoinette MaassenVanDenBrink

摘要

Objective

Understanding age-related changes in migraine is pivotal, considering the increasing global life expectancy. In addition, both aging and migraine are prominent cardiovascular risk factors. It remains unclear whether calcitonin gene-related peptide (CGRP) release changes with age across trigeminovascular components, and how this relates to peripheral responses to migraine-related vasodilatory molecules. The primary aim was to investigate age-related effects on CGRP release from the trigeminovascular system by studying a mouse model of combined accelerated neuronal and vascular aging, the DNA repair-deficient Ercc1Δ/− mice. Second, we assessed the effects of aging on isolated coronary vasodilatory responses to CGRP and forskolin.

Methods

Experiments were conducted using DNA repair-deficient Ercc1Δ/−mice and their wild type controls. After sacrifice, the trigeminal nucleus caudalis (TNC), trigeminal ganglion (TG), and dura mater (DM) were isolated. Ex vivo KCl-induced CGRP release was measured, and CGRP release was compared between Ercc1Δ/− and wild type mice. In a subset of mice, concentration-response curves to CGRP and forskolin were generated in isolated coronary arteries. The pEC50 (negative log of the molar concentration of an agonist needed to reach half of its maximal effect) and Emax (maximum relaxation response) values were compared between both groups.

Results

CGRP release (expressed as ratio compared to baseline release) of the DM was significantly lower in Ercc1Δ/− (2.01 ± 0.24) versus wild type mice (3.22 ± 0.48) (P = 0.040). No differences were observed in CGRP release between Ercc1Δ/−and wild type mice in the TNC (8.07 ± 1.15 versus 6.10 ± 0.57, P = 0.364) or the TG (4.84 ± 0.92 versus 4.41 ± 0.60, P = 0.838). In addition, there were no differences in pEC50 and Emax values in response to CGRP and forskolin.

Conclusion

Our findings suggest that aging is linked to reduced CGRP release of the DM, potentially partly explaining the reduction in migraine attacks in elderly. This decreased CGRP release is not accompanied by altered peripheral vascular reactivity to CGRP.