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Abstract <p>A photoconvertible fluorescent protein based on PAGFP, named PC-mGFP, is developed using molecular evolution in a bacterial system. PC-mGFP undergoes photoconversion from the green fluorescent state with a large Stokes shift to the green fluorescent state when irradiated with 405-nm light. The properties of PC-mGFP are characterized in vitro and in the cytosol of mammalian cells. PC-mGFP can be applied in the field of cellular imaging.</p>

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On the Development of a Genetically Encoded Photoconvertible Green Fluorescent Protein Based on PA-GFP

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月1日

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  • T. P. Kuzmicheva,
  • O. M. Subach,
  • F. V. Subach

摘要

Abstract

A photoconvertible fluorescent protein based on PAGFP, named PC-mGFP, is developed using molecular evolution in a bacterial system. PC-mGFP undergoes photoconversion from the green fluorescent state with a large Stokes shift to the green fluorescent state when irradiated with 405-nm light. The properties of PC-mGFP are characterized in vitro and in the cytosol of mammalian cells. PC-mGFP can be applied in the field of cellular imaging.