Partial Restoration of the Number of Dopaminergic Neurons in the Substantia Nigra in Mice with the Mptp Model of Parkinson’s Disease by Introducing a Microgel Based on Recombinant Spidroin rS1/9

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发表日期：

2025年6月1日

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I. N. Rybolovlev,
M. M. Rudenok,
E. I. Semenova,
M. V. Shulskaya,
S. A. Partevyan,
M. V. Lukashevich,
L. I. Davydova,
M. A. Klinskaya,
V. G. Bogush,
E. L. Arsenyeva,
L. V. Novosadova,
E. V. Novosadova,
M. I. Shadrina,
P. A. Slominsky

摘要

Abstract

It has been previously shown that on a three-dimensional microgel matrix based on recombinant spidroin rS1/9, induced pluripotent stem cells effectively differentiate into a heterogeneous cell population enriched with dopaminergic neurons. An analysis of the functional maturity and activity of the obtained neuronal cultures is carried out, for which they are stereotaxically in vivo administered to mice with a toxin-induced model of Parkinson’s disease. It is shown that the introduction of all analyzed cell-culture variants leads to an increase in the number of dopaminergic neurons and an increase in the proportion of this type of cells in the total cell pool in substantia nigra compacta. This increase is already observed upon the introduction of a microgel based on a mixture of recombinant spidroin proteins rS1/9 and rS2/12 in a ratio of 1 : 1 with 10% heparin-binding peptide and RGDS peptide.