Abstract <p>Ca_{5 –} _<i>x</i>OH(PO₄)₃ hydroxyapatite (HAP) is doped using dysprosium Dy³⁺ and europium Eu³⁺ ions to explore the effect of co-doping by Dy³⁺, Y³⁺ and B³⁺ ions on the luminescent properties of HAP:Eu. The doping involves high-temperature HAP annealing in presence of corresponding Dy₂O₃, Eu₂O₃, Y₂O₃ oxides and boric acid (H₃BO₃). The photoluminescence spectra of doped HAP:Eu and co-doped HAP:Eu, Dy, HAP:Eu, B, HAP:Eu, Y hydroxyapatites are investigated. The continuous laser radiation at the wavelength λ&#xa0;= 405 nm is used to excite photoluminescence. The peculiarities of the change in the photoluminescence spectrum of HAP:Eu after co-doping with Dy³⁺, B³⁺, and Y³⁺ ions are registered. When co-doped using Dy³⁺ ions the HAP:Eu, Dy spectrum consists of narrow spectral peaks corresponding to Dy³⁺ and Eu³⁺ ions in the HAP matrix. When B³⁺, Y³⁺ ions are used for co-doping a dramatic change in the photoluminescence spectra develops: a broad and intense photoluminescence band appears in the HAP:Eu, B and HAP:Eu, Y spectra with the maximum detected at λ = 515 nm and λ = 520 nm, respectively. Co-doping HAP:Dy with B³⁺, Y³⁺ ions practically does not result in changing the photoluminescence spectrum of HAP:Dy consisting of narrow spectral peaks corresponding to Dy³⁺ ions in the HAP matrix.</p>