Cells of Azulene-Containing Plants: Spectral Studies
摘要
The spectral study of the surface and isolated organelles of cells of azulene-containing plants of temperate climate, dry and humid subtropics of Russia was carried out. The presence of azulenes in leaves, flower petals, nuclei and chloroplasts was detected, which was assessed by the appearance of blue color and characteristic maxima in the region of 580–640 nm in the absorption spectra and 405–430 nm in the fluorescence spectra. These data were confirmed experimentally on extracts of these hydrophobic pigments obtained using acetone or ethanol solvents after infusion of intact leaves or isolated organelles (nuclei and chloroplasts) for 10 min (from the surface) or 24 h (also from the surface and from the inside), respectively, as well as after chromatographic purification of the azulene fraction from chlorophyll. The results obtained may be of interest for cellular monitoring of azulene-containing plants of species perspective for pharmacology.