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<b>Abstract</b>— <p>Elucidating the mechanisms of drug resistance in acute myeloid leukemia (AML) cells is a critical endeavor in biomedicine and oncohematology. In our previous research utilizing permanent cell lines, we demonstrated that AML cells in three-dimensional multicellular cultures exhibited increased drug resistance. This study utilized flow cytometry and spectrofluorometry to demonstrate enhanced resistance of primary CD33+ AML cells, cultured in three-dimensional multicellular aggregates, to the cytotoxic effects of anthracyclines. This resistance was associated with the inhibition of the pro-apoptotic signaling pathway, a partial accumulation of cells in the G0/G1 phase of the cell cycle, and an elevation in the levels of the anti-apoptotic protein Bcl-2.</p>

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Resistance of CD33+ Acute Myeloid Leukemia Cells to Anthracyclines in Three-Dimensional Cultures

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • K. S. Krasnov,
  • E. I. Meshcheriakova,
  • Ya. V. Lomovskaya,
  • I. S. Fadeeva,
  • M. I. Kobyakova,
  • R. S. Fadeev

摘要

Abstract—

Elucidating the mechanisms of drug resistance in acute myeloid leukemia (AML) cells is a critical endeavor in biomedicine and oncohematology. In our previous research utilizing permanent cell lines, we demonstrated that AML cells in three-dimensional multicellular cultures exhibited increased drug resistance. This study utilized flow cytometry and spectrofluorometry to demonstrate enhanced resistance of primary CD33+ AML cells, cultured in three-dimensional multicellular aggregates, to the cytotoxic effects of anthracyclines. This resistance was associated with the inhibition of the pro-apoptotic signaling pathway, a partial accumulation of cells in the G0/G1 phase of the cell cycle, and an elevation in the levels of the anti-apoptotic protein Bcl-2.