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Abstract <p><b>Objective:</b> The real-time isothermal Scorpio-DAMP assay for the determination of bacterial pathogens, specifically targeting commensal organisms, is important for clinical practice. <b>Methods:</b> The assay for <i>Neisseria meningitidis</i> was characterized using a set of lower respiratory tract pathogens, with sensitivity assessed at a genomic equivalent (GE) input of 100 000 for a positive reaction, and had no cross-reactivity. <b>Results:</b> The Scorpio-DAMP assay demonstrated a reaction time less than 30 min for positive slope recognition, with negative percent accuracy and positive percent accuracy of 91.8 and 70.6%, respectively. <b>Discussion:</b> Compared to the conventional PCR, which requires temperature cycling and longer processing times, the Scorpio-DAMP assay offers a more accessible solution for high-throughput and low-resource testing environments. <b>Conclusions:</b> The Scorpio-DAMP assay represents a promising alternative for rapid bacterial pathogen detection, particularly suitable for settings with limited resources.</p>

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Specific Detection of Neisseria meningitides by Dual Priming Isothermal Amplification with Scorpion-Shaped Probes

  • 期刊论文

  • 发表日期：

    2025年7月28日

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  • L. A. Shkodenko,
  • V. S. Kuznetsova,
  • M. S. Rubel

摘要

Abstract

Objective: The real-time isothermal Scorpio-DAMP assay for the determination of bacterial pathogens, specifically targeting commensal organisms, is important for clinical practice. Methods: The assay for Neisseria meningitidis was characterized using a set of lower respiratory tract pathogens, with sensitivity assessed at a genomic equivalent (GE) input of 100 000 for a positive reaction, and had no cross-reactivity. Results: The Scorpio-DAMP assay demonstrated a reaction time less than 30 min for positive slope recognition, with negative percent accuracy and positive percent accuracy of 91.8 and 70.6%, respectively. Discussion: Compared to the conventional PCR, which requires temperature cycling and longer processing times, the Scorpio-DAMP assay offers a more accessible solution for high-throughput and low-resource testing environments. Conclusions: The Scorpio-DAMP assay represents a promising alternative for rapid bacterial pathogen detection, particularly suitable for settings with limited resources.