Scholarlink

Abstract <p><b>Objective:</b> The study aimed to investigate morpho-functional changes in the blood-brain barrier (BBB) components of the mouse prefrontal cortex during peripheral tumor growth and after lithium carbonate treatment. <b>Methods:</b> Transmission electron microscopy and immunofluorescence analysis were used to assess BBB alterations, including GFAP and AQP4 expression in astrocytes, as well as volume density of the luminal and basal caveolae, free transport vesicles, and polysomes in prefrontal cortex endothelial cells. Quantitative morphometry and statistical analysis were applied to evaluate significant differences between experimental groups. <b>Results:</b> Tumor-bearing mice exhibited significant BBB disruptions, including a twofold increase in transport vesicle volume density in endothelial cells and increased AQP4-positive astrocytes, despite unchanged GFAP expression. Lithium carbonate treatment maintained AQP4 levels and transport vesicle volume density at levels comparable to control. <b>Conclusions:</b> Peripheral tumor growth increased BBB permeability in the prefrontal cortex, while lithium carbonate treatment correct these structural alterations, suggesting its protective effect on BBB integrity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Disruption of the Blood-Brain Barrier of the Prefrontal Cortex in Peripheral Tumor Growth and Correction with Lithium Carbonate

  • 期刊论文

  • 发表日期：

    2025年7月28日

查看全文
  • N. A. Obanina,
  • N. P. Bgatova

摘要

Abstract

Objective: The study aimed to investigate morpho-functional changes in the blood-brain barrier (BBB) components of the mouse prefrontal cortex during peripheral tumor growth and after lithium carbonate treatment. Methods: Transmission electron microscopy and immunofluorescence analysis were used to assess BBB alterations, including GFAP and AQP4 expression in astrocytes, as well as volume density of the luminal and basal caveolae, free transport vesicles, and polysomes in prefrontal cortex endothelial cells. Quantitative morphometry and statistical analysis were applied to evaluate significant differences between experimental groups. Results: Tumor-bearing mice exhibited significant BBB disruptions, including a twofold increase in transport vesicle volume density in endothelial cells and increased AQP4-positive astrocytes, despite unchanged GFAP expression. Lithium carbonate treatment maintained AQP4 levels and transport vesicle volume density at levels comparable to control. Conclusions: Peripheral tumor growth increased BBB permeability in the prefrontal cortex, while lithium carbonate treatment correct these structural alterations, suggesting its protective effect on BBB integrity.