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Abstract <p> <b>Objectives:</b> Mesenchymal stem/stromal cells (MSC) isolated from equine adipose tissue (AT) represent a promising material for bioveterinary products for the prevention and treatment of many diseases. The production of these cells for clinical use requires improved serum-free culture conditions. The microenvironment can influence the properties of MSC. It is believed that the requirements for culture conditions without animal blood serum are species-specific. The purpose of this study was to evaluate the commercially available serum-free medium (SFM) MesenCult (STEMCELL Technologies, USA), created for human MSC, for the cultivation of equine MSC(AT). <b>Materials and methods:</b> One part of the cells was propagated for 10 passages in the standard DMEM medium with low glucose content (1 g/L) and 10% fetal bovine serum (FBS), and the second in SFM. <b>Results and discussion:</b> The results show that the propagation of equine MSC in MesenCult SFM, intended for the cultivation of human MSC, is possible, since the cells adapt well to it and retain properties characteristic of cells that are cultured in DMEM with FBS: morphology, growth rate, doubling time and mitotic index, clone-forming abilities, diploid set of chromosomes, a large number of cells with the CD90 phenotype (90.8%) and a low number of cells with the CD31 (0.8%), CD34 (0.9%) phenotype, as well as the potency for induction of differentiation into adipo-, osteo- and chondrogenic directions. Equine MSC(AT) showed stable characteristics after being cultured for 10 passages in SFM, providing a promising basis for their further use. <b>Conclusion:</b> Our results demonstrate that MesenCult media may be an alternative for serum-free culture of equine MSC(AT) for expansion in preclinical studies.</p>

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Culture of Equine Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells in Serum-Free Media

  • 期刊论文

  • 发表日期：

    2025年6月25日

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  • I. P. Savchenkova

摘要

Abstract

Objectives: Mesenchymal stem/stromal cells (MSC) isolated from equine adipose tissue (AT) represent a promising material for bioveterinary products for the prevention and treatment of many diseases. The production of these cells for clinical use requires improved serum-free culture conditions. The microenvironment can influence the properties of MSC. It is believed that the requirements for culture conditions without animal blood serum are species-specific. The purpose of this study was to evaluate the commercially available serum-free medium (SFM) MesenCult (STEMCELL Technologies, USA), created for human MSC, for the cultivation of equine MSC(AT). Materials and methods: One part of the cells was propagated for 10 passages in the standard DMEM medium with low glucose content (1 g/L) and 10% fetal bovine serum (FBS), and the second in SFM. Results and discussion: The results show that the propagation of equine MSC in MesenCult SFM, intended for the cultivation of human MSC, is possible, since the cells adapt well to it and retain properties characteristic of cells that are cultured in DMEM with FBS: morphology, growth rate, doubling time and mitotic index, clone-forming abilities, diploid set of chromosomes, a large number of cells with the CD90 phenotype (90.8%) and a low number of cells with the CD31 (0.8%), CD34 (0.9%) phenotype, as well as the potency for induction of differentiation into adipo-, osteo- and chondrogenic directions. Equine MSC(AT) showed stable characteristics after being cultured for 10 passages in SFM, providing a promising basis for their further use. Conclusion: Our results demonstrate that MesenCult media may be an alternative for serum-free culture of equine MSC(AT) for expansion in preclinical studies.