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Abstract <p><b>Objective:</b> Osteoporosis is a disease develops when bone density and quality decreases. Bone tissue is especially sensitive to changes in trace element content in the body. Deficiency of macro- and microelements (silicon, calcium, manganese, etc.) can provoke the development of osteoporosis. The aim of the research was to study the effect of NaBiKat phytoextract on the viability and functional activity of fibroblasts, mesenchymal stem cells (MSC), osteoblasts and osteoclasts, involved in bone metabolism in osteoporosis. <b>Material and methods:</b> An aqueous solution of the NaBiKat phytocomplex consisting of rice husks and germ film, green tea was used in the study; its content of basic chemical elements was measured using atomic immersion spectral analysis. The cytotoxicity of the phytoextract was evaluated on human fibroblasts, MSC and osteoblasts using the MTT test. The activity of mitochondria in cells was assessed by staining with a fluorescent dye TMR. The production of intracellular proteins in osteoblasts and osteoclasts was determined using flow cytometry. <b>Results and discussion:</b> A large amount of sodium, potassium, silicon, manganese, magnesium and a small amount of calcium, iron were found in the aqueous solution of phytoextract. The phytoextract did not exert a cytotoxic effect on fibroblasts and stimulated proliferation up to 112–128 % in MSC and up to 130 % in osteoblasts. At maximum concentration, the phytocomplex reduced osteoblast proliferation. Phytocomplex enhanced mitochondria activity in MSC and osteoblasts, reduced it in fibroblasts and osteoclasts. Under its influence, increased RANKL synthesis was observed in osteoblasts, the amount of type 1 collagen did not change, and cathepsin K production decreased in osteoclasts, while it did not significantly affect MMP-9 content. <b>Conclusions:</b> The results obtained show that the aqueous phytoextract based on rice husk and germ film, green tea leaves is safe for MSC, fibroblasts, osteoblasts, and osteoclasts in humans and is able to stimulate the functional activity of osteoblasts, as well as reduce the activity of osteoclasts due to the high content of macro- and microelements.</p>

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Effect of Phytoextract Based on Rice Husk and Germ Film, Green Tea on the Functional Properties of Cells Involved in Bone Tissue Metabolism

  • 期刊论文

  • 发表日期：

    2025年2月10日

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  • N. A. Sitnikova,
  • N. A. Bondarenko,
  • A. O. Kushnarenko

摘要

Abstract

Objective: Osteoporosis is a disease develops when bone density and quality decreases. Bone tissue is especially sensitive to changes in trace element content in the body. Deficiency of macro- and microelements (silicon, calcium, manganese, etc.) can provoke the development of osteoporosis. The aim of the research was to study the effect of NaBiKat phytoextract on the viability and functional activity of fibroblasts, mesenchymal stem cells (MSC), osteoblasts and osteoclasts, involved in bone metabolism in osteoporosis. Material and methods: An aqueous solution of the NaBiKat phytocomplex consisting of rice husks and germ film, green tea was used in the study; its content of basic chemical elements was measured using atomic immersion spectral analysis. The cytotoxicity of the phytoextract was evaluated on human fibroblasts, MSC and osteoblasts using the MTT test. The activity of mitochondria in cells was assessed by staining with a fluorescent dye TMR. The production of intracellular proteins in osteoblasts and osteoclasts was determined using flow cytometry. Results and discussion: A large amount of sodium, potassium, silicon, manganese, magnesium and a small amount of calcium, iron were found in the aqueous solution of phytoextract. The phytoextract did not exert a cytotoxic effect on fibroblasts and stimulated proliferation up to 112–128 % in MSC and up to 130 % in osteoblasts. At maximum concentration, the phytocomplex reduced osteoblast proliferation. Phytocomplex enhanced mitochondria activity in MSC and osteoblasts, reduced it in fibroblasts and osteoclasts. Under its influence, increased RANKL synthesis was observed in osteoblasts, the amount of type 1 collagen did not change, and cathepsin K production decreased in osteoclasts, while it did not significantly affect MMP-9 content. Conclusions: The results obtained show that the aqueous phytoextract based on rice husk and germ film, green tea leaves is safe for MSC, fibroblasts, osteoblasts, and osteoclasts in humans and is able to stimulate the functional activity of osteoblasts, as well as reduce the activity of osteoclasts due to the high content of macro- and microelements.