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Abstract <p><b>Objective:</b> Currently, the actual issue now issues is the quantitative assessment of the isolated effects of physical and chemical occupational factors of low intensity, severity and intensity of the labor process on the telomere relative length (TRL) to clarify the mechanisms of development of premature aging. The aim was to evaluate the effect of isolated exposure to low–intensity physical and chemical factors od production on the relative length of telomeres in an animal experiment. <b>Material and methods:</b> ICR mice (<i>n</i> = 65) are distributed in equal numbers into 3 test groups (exposure to vibration, noise, chemical exposure) and the control group (contained in comfortable conditions and not exposed to these factors). The duration of the experiment was 90 days, samples for DNA isolation from the striated muscle tissue of ICR mice thigh were taken on days 0, 30, 60 and 90. TRL was measured using real time PCR. <b>Results and discussion:</b> The results of the study indicate a significant shortening of TRL on the 90th day in the control group compared to the initial indicators, which may indicate the general processes of aging of animals. A long (90-day) exposure of ICR mice to conditions simulating the isolated influence of various hazard factors of production (vibration, noise, chemicals) operating at 1.5 MAC, MAL, was accompanied by unidirectional dynamics of TRL. The chemical factor had the greatest impact on TRL (a decrease in TRL relative to the value of the control group was noted on the 30th, 60th and 90th days of the experiment), the physical factor caused a shortening of TRL after 60 and 90 days. <b>Conclusions:</b> Further study of the isolated influence of l factors of production on the TRL changes in model organisms will contribute to clarify the mechanisms that prevent the development of pathological processes in workers exposed to occupational hazards.</p>

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Effect of Production Factors on the Relative Telomere Length of ICR Mice

  • 期刊论文

  • 发表日期：

    2025年2月10日

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  • O. A. Savchenko,
  • P. E. Svechkar,
  • I. I. Novikova

摘要

Abstract

Objective: Currently, the actual issue now issues is the quantitative assessment of the isolated effects of physical and chemical occupational factors of low intensity, severity and intensity of the labor process on the telomere relative length (TRL) to clarify the mechanisms of development of premature aging. The aim was to evaluate the effect of isolated exposure to low–intensity physical and chemical factors od production on the relative length of telomeres in an animal experiment. Material and methods: ICR mice (n = 65) are distributed in equal numbers into 3 test groups (exposure to vibration, noise, chemical exposure) and the control group (contained in comfortable conditions and not exposed to these factors). The duration of the experiment was 90 days, samples for DNA isolation from the striated muscle tissue of ICR mice thigh were taken on days 0, 30, 60 and 90. TRL was measured using real time PCR. Results and discussion: The results of the study indicate a significant shortening of TRL on the 90th day in the control group compared to the initial indicators, which may indicate the general processes of aging of animals. A long (90-day) exposure of ICR mice to conditions simulating the isolated influence of various hazard factors of production (vibration, noise, chemicals) operating at 1.5 MAC, MAL, was accompanied by unidirectional dynamics of TRL. The chemical factor had the greatest impact on TRL (a decrease in TRL relative to the value of the control group was noted on the 30th, 60th and 90th days of the experiment), the physical factor caused a shortening of TRL after 60 and 90 days. Conclusions: Further study of the isolated influence of l factors of production on the TRL changes in model organisms will contribute to clarify the mechanisms that prevent the development of pathological processes in workers exposed to occupational hazards.