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Abstract <p><b>Objective:</b> The risk of developing mammary cancer increases with a history of non-malignant mammary diseases (NMMD). Therefore, it seems relevant to search for criteria for cell malignancy in NMMD. The aim of the study is to monitor the association between the expression of proliferation markers, epithelial-mesenchymal transition (EMT) and histidine-rich glycoprotein (HRG) mRNA in NMMD. <b>Material and methods:</b> In breast tissue biopsies of 37 patients with invasive carcinoma of no special type (ICNST) and 17 patients with NMMD, the expression of proliferation markers (Ki-67 and cyclin (CCND1)), EMT markers (E-cadherin (CDH1), type II collagen (CII) and β1-integrin (CD29)) was determined by immunohistochemistry. HRG mRNA expression was assessed by real-time PCR. <b>Results:</b> Expression of HRG mRNA was detected in 91.9% of cases (34 of 37) in ICNST and in 82.4% (14 of 17) in NMMD, and in the latter case it was inversely related to the expression of CDH1, CD29, and Ki-67. A direct relationship was established between the representation of Ki-67 and CCND1, CII, and between CCND1 and CD29 in NMMD. In patients with ICNST, a inverse correlation was found between the expression of HRG mRNA and the presence of CII, and an direct correlation was found between the number of cells containing CII and CD29. It was found that in ICNST and NMMD with the presence of HRG mRNA expression, the representation of CDH1 was lower than in its absence. <b>Conclusion:</b> HRG mRNA expression indicators in NMMD in combination with the assessment of the presence of proliferation markers and EMT can be useful in developing criteria for the probable malignancy of cells in NMMD.</p>

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Markers of Proliferation and Epithelial-Mesenchymal Transition and Their Association with Histidine-Rich Glycoprotein HRG mRNA Expression in Breast Diseases

  • 期刊论文

  • 发表日期：

    2025年1月20日

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  • S. A. Arkhipov,
  • A. A. Studenikina,
  • V. V. Arkhipova,
  • A. V. Proskura,
  • A. I. Autenshlyus

摘要

Abstract

Objective: The risk of developing mammary cancer increases with a history of non-malignant mammary diseases (NMMD). Therefore, it seems relevant to search for criteria for cell malignancy in NMMD. The aim of the study is to monitor the association between the expression of proliferation markers, epithelial-mesenchymal transition (EMT) and histidine-rich glycoprotein (HRG) mRNA in NMMD. Material and methods: In breast tissue biopsies of 37 patients with invasive carcinoma of no special type (ICNST) and 17 patients with NMMD, the expression of proliferation markers (Ki-67 and cyclin (CCND1)), EMT markers (E-cadherin (CDH1), type II collagen (CII) and β1-integrin (CD29)) was determined by immunohistochemistry. HRG mRNA expression was assessed by real-time PCR. Results: Expression of HRG mRNA was detected in 91.9% of cases (34 of 37) in ICNST and in 82.4% (14 of 17) in NMMD, and in the latter case it was inversely related to the expression of CDH1, CD29, and Ki-67. A direct relationship was established between the representation of Ki-67 and CCND1, CII, and between CCND1 and CD29 in NMMD. In patients with ICNST, a inverse correlation was found between the expression of HRG mRNA and the presence of CII, and an direct correlation was found between the number of cells containing CII and CD29. It was found that in ICNST and NMMD with the presence of HRG mRNA expression, the representation of CDH1 was lower than in its absence. Conclusion: HRG mRNA expression indicators in NMMD in combination with the assessment of the presence of proliferation markers and EMT can be useful in developing criteria for the probable malignancy of cells in NMMD.