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Abstract <p><b>Objective:</b> The presented review considers in a comparative key the role of organism systems in karyotypic variability, the influence of various environmental factors on the karyotypic structure of cells of markerless (mainly immortalized) cell lines during long-term cultivation. <b>Methods:</b> Special attention is paid to the characteristics of dicentrics based on telomeric associations (D-TA). In addition, data on the karyotypic characteristics of various non-immortalized markerless lines of human mesenchymal stem cells are presented. <b>Results and Discussion:</b> Certain properties of D-TA and the relationship between quantitative (SVK) and structural (D-TA) karyotypic variability in markerless cell lines are discussed. <b>Conclusions:</b> The analysis allowed to draw the following conclusion: 1) D-TA are, apparently, genetic structures providing adaptation of the cell population, as an autonomous formation, to the conditions of existence <i>in vitro</i>, i.e. to the elimination of defects of the genetic apparatus that arise when the conditions of cell vital activity change; 2) the relationship between quantitative and structural karyotypic variability is a manifestation of a uniform cell-population function; 3) a comparison of structural karyotypic variability in different marker-free cell lines in response to external inducing effects and in the process of replicative aging (RS) suggests that cell changes in the process of RS are only a partial factor contributing to the generation of D-TA.</p>

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Karyotypic Variability in Different Marker-Free Cell Lines

  • 期刊论文

  • 发表日期：

    2025年1月20日

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  • G. G. Poljanskaya,
  • V. I. Turilova,
  • T. K. Yakovleva

摘要

Abstract

Objective: The presented review considers in a comparative key the role of organism systems in karyotypic variability, the influence of various environmental factors on the karyotypic structure of cells of markerless (mainly immortalized) cell lines during long-term cultivation. Methods: Special attention is paid to the characteristics of dicentrics based on telomeric associations (D-TA). In addition, data on the karyotypic characteristics of various non-immortalized markerless lines of human mesenchymal stem cells are presented. Results and Discussion: Certain properties of D-TA and the relationship between quantitative (SVK) and structural (D-TA) karyotypic variability in markerless cell lines are discussed. Conclusions: The analysis allowed to draw the following conclusion: 1) D-TA are, apparently, genetic structures providing adaptation of the cell population, as an autonomous formation, to the conditions of existence in vitro, i.e. to the elimination of defects of the genetic apparatus that arise when the conditions of cell vital activity change; 2) the relationship between quantitative and structural karyotypic variability is a manifestation of a uniform cell-population function; 3) a comparison of structural karyotypic variability in different marker-free cell lines in response to external inducing effects and in the process of replicative aging (RS) suggests that cell changes in the process of RS are only a partial factor contributing to the generation of D-TA.