Scholarlink

Abstract <p>Effective approaches for selecting nuclei in which a gene of interest is in an active state are necessary for studying spatial organization and gene expression regulation. In this work, a novel two-component genetic system was created for the parasegment-specific labeling of nuclei in which the regulatory domain <i>iab-5</i>, which stimulates the homeotic gene <i>Abd-B</i>, is active. The system is based on the integration of a transgene expressing the yeast GAL4 activator under the control of the minimal <i>hsp70</i> gene promoter into the <i>iab-5</i> domain, near the early embryonic enhancer. As a result, the <i>iab-5</i> enhancer induces specific expression of GAL4, which strongly amplifies the expression of the <i>mScarlet</i> fluorescent protein, allowing for the efficient selection of labeled nuclei in which the <i>iab-5</i> domain is activated. This approach can be used for the selection of target nuclei in which any regulatory element or gene of interest is in an active or repressed state.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development of an Approach for Parasegment-Specific Labeling of Nuclei in Drosophila melanogaster Embryos

  • 期刊论文

  • 发表日期：

    2026年4月20日

查看全文
  • I. O. Deriglazova,
  • O. V. Kyrchanova,
  • P. G. Georgiev,
  • O. G. Maksimenko

摘要

Abstract

Effective approaches for selecting nuclei in which a gene of interest is in an active state are necessary for studying spatial organization and gene expression regulation. In this work, a novel two-component genetic system was created for the parasegment-specific labeling of nuclei in which the regulatory domain iab-5, which stimulates the homeotic gene Abd-B, is active. The system is based on the integration of a transgene expressing the yeast GAL4 activator under the control of the minimal hsp70 gene promoter into the iab-5 domain, near the early embryonic enhancer. As a result, the iab-5 enhancer induces specific expression of GAL4, which strongly amplifies the expression of the mScarlet fluorescent protein, allowing for the efficient selection of labeled nuclei in which the iab-5 domain is activated. This approach can be used for the selection of target nuclei in which any regulatory element or gene of interest is in an active or repressed state.