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Abstract <p>Malectin and malectin-like proteins (M/MLPs) form one of the largest plant lectin families, yet the carbohydrate specificity of most members remains largely unexplored. FERONIA, a receptor-like kinase and key regulator of cell expansion, is the best-known member experimentally confirmed to bind pectins. Using transcriptomic data from plant species with contrasting cell wall pectin contents<i>—Arabidopsis thaliana</i>, maize (<i>Zea mays</i>), celery (<i>Apium graveolens</i>), and flax (<i>Linum usitatissimum</i>)—we revised all <i>M</i>/<i>MLP</i> genes and analyzed their expression profiles, alongside genes encoding glycosyltransferases (GTs) involved in cell wall polysaccharide biosynthesis. Distinct sets of <i>M/MLPs</i> were upregulated during cell expansion, in pectin-rich tissues, and during wall thickening, with only partial overlap between orthologs of monocots and dicots. A strong coexpression of <i>M</i>/<i>MLP</i> genes with those encoding GTs associated with pectin biosynthesis was found in celery, whereas <i>M</i>/<i>MLP</i>s expressed in flax fibers with tertiary walls exhibited little correlation. In contrast, almost all <i>M</i>/<i>MLP</i> genes in all analyzed samples showed no or negative expression correlation with genes encoding GTs for xyloglucan and cellulose biosynthesis. These results indicate functional specialization within the M/MLP family and highlight candidate genes for future studies on pectin recognition and cell wall sensing.</p>

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Beyond FERONIA: Gene Expression Profiling Reveals Functional Diversification of Malectin/Malectin-Like Proteins in Tissues with Cell Walls Differing in Pectin Content

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • N. Syrchina,
  • N. Mokshina,
  • N. Petrova,
  • A. Aglyamova,
  • N. Shilova,
  • T. Gorshkova

摘要

Abstract

Malectin and malectin-like proteins (M/MLPs) form one of the largest plant lectin families, yet the carbohydrate specificity of most members remains largely unexplored. FERONIA, a receptor-like kinase and key regulator of cell expansion, is the best-known member experimentally confirmed to bind pectins. Using transcriptomic data from plant species with contrasting cell wall pectin contents—Arabidopsis thaliana, maize (Zea mays), celery (Apium graveolens), and flax (Linum usitatissimum)—we revised all M/MLP genes and analyzed their expression profiles, alongside genes encoding glycosyltransferases (GTs) involved in cell wall polysaccharide biosynthesis. Distinct sets of M/MLPs were upregulated during cell expansion, in pectin-rich tissues, and during wall thickening, with only partial overlap between orthologs of monocots and dicots. A strong coexpression of M/MLP genes with those encoding GTs associated with pectin biosynthesis was found in celery, whereas M/MLPs expressed in flax fibers with tertiary walls exhibited little correlation. In contrast, almost all M/MLP genes in all analyzed samples showed no or negative expression correlation with genes encoding GTs for xyloglucan and cellulose biosynthesis. These results indicate functional specialization within the M/MLP family and highlight candidate genes for future studies on pectin recognition and cell wall sensing.