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Abstract <p>The autonomous functioning of regulatory domains in the Bithorax complex (BX-C) of <i>Drosophila melanogaster</i> is maintained by boundaries (insulators) that prevent inappropriate enhancer–promoter interactions. Polycomb response elements (PREs) maintain epigenetic memory within regulatory domains and are often located near insulators, enhancing their blocking activity. The <i>bxd</i>&#xa0;PRE is a well-characterized silencer that regulates the <i>bxd</i>/<i>pbx</i> domain of the <i>Ubx</i> gene in the first abdominal segment. We used a boundary replacement strategy to assess the insulator activity of the <i>bxd</i>&#xa0;PRE. Substituting the <i>bxd</i>&#xa0;PRE for the <i>Fab-</i>7 boundary, which separates the <i>Abd-B</i> regulatory domains in the BX-C, revealed that the <i>bxd</i>&#xa0;PRE has weak insulator activity.</p>

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The bxd PRE from Bithorax Complex of Drosophila melanogaster Has Weak Insulator Activity

  • 期刊论文

  • 发表日期：

    2025年4月11日

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  • A. N. Ibragimov,
  • O. V. Kyrchanova,
  • Y. E. Vorontsova,
  • E. N. Kozlov,
  • V. O. Dubrovskaya,
  • P. G. Georgiev

摘要

Abstract

The autonomous functioning of regulatory domains in the Bithorax complex (BX-C) of Drosophila melanogaster is maintained by boundaries (insulators) that prevent inappropriate enhancer–promoter interactions. Polycomb response elements (PREs) maintain epigenetic memory within regulatory domains and are often located near insulators, enhancing their blocking activity. The bxd PRE is a well-characterized silencer that regulates the bxd/pbx domain of the Ubx gene in the first abdominal segment. We used a boundary replacement strategy to assess the insulator activity of the bxd PRE. Substituting the bxd PRE for the Fab-7 boundary, which separates the Abd-B regulatory domains in the BX-C, revealed that the bxd PRE has weak insulator activity.