Scholarlink

Abstract <p>This study was designed to evaluate the effect of Dendrobiumofficinale (DO) extract on the PI3K/Akt signaling cascade in a murinemodel of ulcerative colitis (UC) and to explore the underlying mechanisms.For this sixty male BALB/c mice with comparable weights were randomlydivided into six groups: a normal control, a DSS-induced UC model,a positive control treated with 5-aminosalicylic acid (5-ASA), andthree groups receiving DO extract at low (200 mg·kg<sup>–1</sup>),medium (400 mg·kg<sup>–1</sup>), and high (800 mg·kg<sup>–1</sup>)doses, with 10 mice per group. The effects of DO extract on bodyweight and colon length were observed, as well as its impact ondisease activity index (DAI) score, serum cytokine (IL-1β, IL-6,TNF-α) levels, histopathological changes in the colon, and the expressionof phosphorylated PI3K and Akt proteins through western blot analysis.It was found that DSS-induced UC mice exhibited higher DAI scores,IL-1β, IL-6, and TNF-α levels, and histopathological injury scores.After treatment, DO extract at different doses reduced DAI scoresand histopathological injury scores, lowered serum IL-1β, IL-6,and TNF-α levels, and decreased the expression of p-PI3K and p-Aktproteins, leading to improvement of colon injury. This study suggeststhat DO therapeutic effects on DSS-induced UC mice may exert itseffects via the PI3K/Akt pathway.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effect of Dendrobium Officinale Extract on the PI3K/Akt Pathway in Mice with Ulcerative Colitis and Its Mechanism of Action

  • 期刊论文

  • 发表日期：

    2025年10月1日

查看全文
  • Mingwen Jia,
  • Zifu Hong,
  • Yufei Li,
  • E Li,
  • Ang Li,
  • Yiping Li,
  • Zhen Liu,
  • Muwen Qu

摘要

Abstract

This study was designed to evaluate the effect of Dendrobiumofficinale (DO) extract on the PI3K/Akt signaling cascade in a murinemodel of ulcerative colitis (UC) and to explore the underlying mechanisms.For this sixty male BALB/c mice with comparable weights were randomlydivided into six groups: a normal control, a DSS-induced UC model,a positive control treated with 5-aminosalicylic acid (5-ASA), andthree groups receiving DO extract at low (200 mg·kg–1),medium (400 mg·kg–1), and high (800 mg·kg–1)doses, with 10 mice per group. The effects of DO extract on bodyweight and colon length were observed, as well as its impact ondisease activity index (DAI) score, serum cytokine (IL-1β, IL-6,TNF-α) levels, histopathological changes in the colon, and the expressionof phosphorylated PI3K and Akt proteins through western blot analysis.It was found that DSS-induced UC mice exhibited higher DAI scores,IL-1β, IL-6, and TNF-α levels, and histopathological injury scores.After treatment, DO extract at different doses reduced DAI scoresand histopathological injury scores, lowered serum IL-1β, IL-6,and TNF-α levels, and decreased the expression of p-PI3K and p-Aktproteins, leading to improvement of colon injury. This study suggeststhat DO therapeutic effects on DSS-induced UC mice may exert itseffects via the PI3K/Akt pathway.