Abstract <p>The Anna gold deposit is a new industrial facility explored between 2006 and 2016 within the oldest Nevyansk–Berezovsky gold-bearing district of the Middle Urals. It is located 45 km north of Yekaterinburg and is confined to the subvertical Verkhotursky meridional oblique-slip fault, occurring among meta-ultrabasic rocks of the Pervomaysky massif. The latter has an arched anticline shape and is cut by some Late Paleozoic granitoid intrusions. The weakly gold-bearing sulfide mineralization of the deposit is associated with quartz veinlets in listvenites. It is represented by native gold, pyrite, chalcopyrite, galena, and fahlore. The productive mineralization of the deposit is associated with a columnar, steeply dipping eastward body of quartz metasomatites (quartz &gt;90%) of the quartz-sericite formation, developed after listvenite and talc–carbonate rocks. The main ore minerals are gold-bearing pyrite and arsenopyrite. The measured gold content in pyrite reaches 800 g/t, and in arsenopyrite–1200 g/t. Such concentrations are typical for deposits with “invisible” gold. The low-grade gold-bearing listvenites of the deposit were formed from waters with δ¹⁸O H₂O = +7.8 + 12.0‰ and δ¹³С CO₂ = –6.8 to –10.3‰ at a temperature of 359 ± 21°C, pressure of 1.84–1.58 kbar, and salinity of 11–17% in NaCl eq. The calculated isotopic composition δ¹⁸O H₂O of the fluid in the productive quartz-sericite metasomatites of the deposit is 5.4 ± 0.4‰. The homogenization temperature of fluid inclusions in quartz is 272 ± 7°C, pressure is 0.61–0.87 kbar, and salinity is 2.5–3.4% in NaCl eq. These data suggest the involvement of magmatogenic fluid in the formation of early non-ore propylites and low-grade gold-bearing listvenites, and mixed fluid, with the participation of metamorphic or meteoric origin, in the formation of productive quartz–sericite metasomatites during the acid leaching stage.</p>