Abstract <p>The biosynthetic process, also known as green synthesis, is particularly attractive due to its ability to reduce nanoparticle toxicity. Vitamins, amino acids, and plant extracts are currently gaining widespread popularity. Conversely, this technique creates nanoparticles by utilizing reducing agents derived from plants and bacteria. It has various advantages, including non-toxicity, environmental safety, cost-effectiveness, biocompatibility, and energy generation. Nanoparticle manufacturing can involve a diverse range of components. Using grape leaf extract as a raw material, we have created an affordable and environmentally safe way to produce titanium dioxide nanoparticles (GL-TiO₂ NPs) from a titanium isopropoxide solution. Nanoparticles were examined by using X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), Brunauer‒Emmett‒Teller (BET) analysis, with particle size analysis (PSA), Energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), and Zeta potential analysis. The distinct peaks observed in the XRD pattern demonstrate the crystalline structure and level of quality of titanium dioxide (TiO₂) nanoparticles. The form and structure were examined by FE-SEM analysis, and EDS examinations. Data indicate that the size of the nanoparticle vary between 22 and 49 nm. The size distribution of the TiO₂ nanoparticles synthesized by a green method has been determined by utilizing a particle size analyzer (PSA) and XRD to measure the average crystalline size. The surface area of the created particles was determined using BET. The analysis revealed a specific surface area of 50.4716 m²/g. This paper underscored the advantages of utilizing nanoparticles in nanotechnology without any adverse impacts.</p>