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Abstract <p><b>Objective:</b> Lactoferrin is a multifunctional glycoprotein with beneficial properties, including immunomodulatory, anti-inflammatory, antibacterial, antiviral and antioxidant properties. hLF contained in breast milk provides natural protection of newborns from infections. Testing the quality of breast milk and colostrum is an important task that will enable the adjustment of the baby’s nutrition. A large number of methods for determining hLF are known, most often instrumental and immune tests are used. However, simple and rapid analysis methods are needed. <b>Methods:</b> 32 colostrum samples and 10 milk were obtained from healthy women. Lactoferrin content was tested by fluorescence polarization immunoassay (FPIA) and ELISA using monoclonal and polyclonal antibodies. <b>Results and Discussion:</b> Previously, we demonstrated the possibility of determining hLF by the FPIA using fluorescently labeled recombinant camel nanobodies, which took no more than 10 min. In this work, we demonstrate the possibility of determining hLF in colostrum samples, which differs in composition from milk. A one-step enzyme immunoassay using polyclonal and monoclonal antibodies has been developed, which allows for faster and minimal-stage determination of hLF in milk and colostrum. The ELISA detection limit was 1.25 ng/mL. 32 colostrum samples and 10 milk samples were tested using FPIA and ELISA. <b>Conclusions:</b> It has been shown that both methods correctly determine hLF in milk and colostrum. It is important to note that the developed ELISA can be extended to other human physiological fluids, such as tear fluid or urine.</p>

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Prospects for Determining Lactoferrin in Human Milk and Colostrum Using Immunological Diagnostic Methods

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • L. I. Mukhametova,
  • S. A. Eremin,
  • M. K. Kolokolova,
  • Yu. S. Lebedin,
  • I. V. Mikhura,
  • O. V. Stetsiuk,
  • T. I. Ivanova,
  • S. V. Tillib

摘要

Abstract

Objective: Lactoferrin is a multifunctional glycoprotein with beneficial properties, including immunomodulatory, anti-inflammatory, antibacterial, antiviral and antioxidant properties. hLF contained in breast milk provides natural protection of newborns from infections. Testing the quality of breast milk and colostrum is an important task that will enable the adjustment of the baby’s nutrition. A large number of methods for determining hLF are known, most often instrumental and immune tests are used. However, simple and rapid analysis methods are needed. Methods: 32 colostrum samples and 10 milk were obtained from healthy women. Lactoferrin content was tested by fluorescence polarization immunoassay (FPIA) and ELISA using monoclonal and polyclonal antibodies. Results and Discussion: Previously, we demonstrated the possibility of determining hLF by the FPIA using fluorescently labeled recombinant camel nanobodies, which took no more than 10 min. In this work, we demonstrate the possibility of determining hLF in colostrum samples, which differs in composition from milk. A one-step enzyme immunoassay using polyclonal and monoclonal antibodies has been developed, which allows for faster and minimal-stage determination of hLF in milk and colostrum. The ELISA detection limit was 1.25 ng/mL. 32 colostrum samples and 10 milk samples were tested using FPIA and ELISA. Conclusions: It has been shown that both methods correctly determine hLF in milk and colostrum. It is important to note that the developed ELISA can be extended to other human physiological fluids, such as tear fluid or urine.