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Abstract <p>Research on stem cell differentiation increasingly focuses on the vesicular paracrine component, which facilitates intercellular communication and defines cellular functional status. This study compares the content (mRNA and proteins) of extracellular vesicles (EVs) from human induced pluripotent stem cells (iPSCs) with the molecular profile of the cells themselves during directed neural differentiation. We found that changes in EV content mirrored the cell differentiation trajectory: expression of pluripotency marker genes (<i>Oct4, Sox2, cMyc, Nanog</i>) decreased, while markers of neural differentiation (<i>Pax6, Nestin, Tubb, Map2, S100B, GFAP</i>) appeared. Mass spectrometry analysis revealed a functional diversification in the EV proteome, reflecting various stages of differentiation—from regulatory processes to the functional specification of neural derivatives.</p>

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Changes in the Composition of Extracellular Vesicles during Neural Differentiation of Pluripotent Stem Cells

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • E. A. Sazonova,
  • E. A. Suprunenko,
  • E. G. Evtushenko,
  • E. A. Evtushenko,
  • A. V. Vasiliev

摘要

Abstract

Research on stem cell differentiation increasingly focuses on the vesicular paracrine component, which facilitates intercellular communication and defines cellular functional status. This study compares the content (mRNA and proteins) of extracellular vesicles (EVs) from human induced pluripotent stem cells (iPSCs) with the molecular profile of the cells themselves during directed neural differentiation. We found that changes in EV content mirrored the cell differentiation trajectory: expression of pluripotency marker genes (Oct4, Sox2, cMyc, Nanog) decreased, while markers of neural differentiation (Pax6, Nestin, Tubb, Map2, S100B, GFAP) appeared. Mass spectrometry analysis revealed a functional diversification in the EV proteome, reflecting various stages of differentiation—from regulatory processes to the functional specification of neural derivatives.