Scholarlink

Abstract <p>In this study, a comparative computational approach was done to analyse and annotate 100 hypothetical proteins (HPs) derived from <i>Oryza barthii</i> (African rice), a member of the Poaceae family. These proteins, whose biochemical, molecular and cellular functions remain uncharacterized, known as HPs, were retrieved from the NCBI database. Several functional annotations were done utilizing the protein sequences to characterize the role of individual protein in physiology of plant systems. The analysis encompassed study of protein structure, function, subcellular locations, and interacting partners, in addition to predicting orthologous relationships. The majority of proteins were predicted to function as stress response proteins. Subcellular localization plays a crucial role for accurately localization of identifying proteins, revealing their cellular mechanisms. Signal peptide’s prevalence can also be anticipated. Most of the proteins had orthologous members closest to the protein sequence of <i>Oryza glaberrima</i> and few proteins had their closest orthologous members belonging to the protein sequence of other <i>Oryza</i> species like, <i>O. glumipetula</i>, <i>O. nivara</i>, etc. Functional analysis along with the secondary structure assessment of these HPs highlighted the involvement of some proteins in both biotic and abiotic stress management, and in defence response in plants. Hypothetical proteins have the potential to provide insight into various unidentified protein structures and functionalities, making them a promising area for advanced research.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Explication of Hypothetical Proteins from Oryza barthii A. Chev. Proteomes by Comparative Assessment for Structural Analysis of Developing New Proteins

  • 期刊论文

  • 发表日期：

    2025年8月25日

查看全文
  • Arpita Banerjee,
  • Patrali Chakraborty,
  • Bidisha Mallick,
  • Dipu Samanta,
  • Debleena Roy

摘要

Abstract

In this study, a comparative computational approach was done to analyse and annotate 100 hypothetical proteins (HPs) derived from Oryza barthii (African rice), a member of the Poaceae family. These proteins, whose biochemical, molecular and cellular functions remain uncharacterized, known as HPs, were retrieved from the NCBI database. Several functional annotations were done utilizing the protein sequences to characterize the role of individual protein in physiology of plant systems. The analysis encompassed study of protein structure, function, subcellular locations, and interacting partners, in addition to predicting orthologous relationships. The majority of proteins were predicted to function as stress response proteins. Subcellular localization plays a crucial role for accurately localization of identifying proteins, revealing their cellular mechanisms. Signal peptide’s prevalence can also be anticipated. Most of the proteins had orthologous members closest to the protein sequence of Oryza glaberrima and few proteins had their closest orthologous members belonging to the protein sequence of other Oryza species like, O. glumipetula, O. nivara, etc. Functional analysis along with the secondary structure assessment of these HPs highlighted the involvement of some proteins in both biotic and abiotic stress management, and in defence response in plants. Hypothetical proteins have the potential to provide insight into various unidentified protein structures and functionalities, making them a promising area for advanced research.