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Abstract <p>The present article is devoted to optimizing rooting of <i>Cattleya gaskelliana</i> regenerants in vitro, and to identifying anatomical features of root under various culture conditions in vitro. The results showed that semi-solid 1/2 MS nutrient medium containing 50.0 g/L banana puree and 1.0 mg/L IAA is more effective in the growth and rooting of C. <i>gaskelliana</i> regenerants. Also, it has been determined the histological differentiation of <i>C. gaskelliana</i> roots formed on nutrient media with different concentrations of agar. Plants cultured on semi-solid nutrient medium had roots with more developed diameter and a central cylinder. To obtain maximum survival rate (100%), it is recommended to use sphagnum moss and a substrate consisting of bark, perlite, and peat in equal ratio.</p>

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Improving Rooting of Cattleya gaskelliana (N.E. Br.) B.S. Williams (Orchidaceae Juss.) under In Vitro Conditions

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • Muthab Hussien,
  • O. I. Molkanova,
  • E. E. Orlova,
  • V. A. Koval

摘要

Abstract

The present article is devoted to optimizing rooting of Cattleya gaskelliana regenerants in vitro, and to identifying anatomical features of root under various culture conditions in vitro. The results showed that semi-solid 1/2 MS nutrient medium containing 50.0 g/L banana puree and 1.0 mg/L IAA is more effective in the growth and rooting of C. gaskelliana regenerants. Also, it has been determined the histological differentiation of C. gaskelliana roots formed on nutrient media with different concentrations of agar. Plants cultured on semi-solid nutrient medium had roots with more developed diameter and a central cylinder. To obtain maximum survival rate (100%), it is recommended to use sphagnum moss and a substrate consisting of bark, perlite, and peat in equal ratio.