Scholarlink

Abstract <p>A new electrochemical approach to enzyme-linked immunosorbent assay (ELISA) on magnetic microparticles functionalized with antibodies specific to the cardiac biomarker NT-proBNP is described. Alkaline phosphatase was selected as the enzyme label; the signal was detected by differential pulse voltammetry on disposable electrodes. The method offers high sensitivity, with a limit of detection of ~35 pg/mL, and a short analysis time of down to 20 min, which makes it promising for clinical applications. The key parameters, including component concentrations, temperature, and incubation time, were optimized. The procedure was validated on 34 serum samples from healthy individuals and patients with cardiovascular diseases. To assess clinical applicability, a correlation analysis was performed between the electrochemical signal intensities and the concentrations obtained on the Mindray BS-800 and Getein-1100 analyzers. Statistical analysis showed strong linear correlations between the signal obtained by the developed procedure and the target protein concentrations in the samples: <i>r</i> = 0.96 with the Mindray BS-800 and <i>r</i> = 0.93 with the Getein-1100.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

ELISA on Magnetic Microparticles with Electrochemical Detection for NT-proBNP Analysis in Human Serum

  • 期刊论文

  • 发表日期：

    2025年11月11日

查看全文
  • O. N. Sorokina,
  • T. S. Konstantinova,
  • A. K. Vorobyova,
  • A. D. Vasilyeva,
  • A. V. Eremenko,
  • A. V. Lyzhenkova,
  • E. V. Zobova,
  • L. O. Minushkina,
  • I. N. Kurochkin

摘要

Abstract

A new electrochemical approach to enzyme-linked immunosorbent assay (ELISA) on magnetic microparticles functionalized with antibodies specific to the cardiac biomarker NT-proBNP is described. Alkaline phosphatase was selected as the enzyme label; the signal was detected by differential pulse voltammetry on disposable electrodes. The method offers high sensitivity, with a limit of detection of ~35 pg/mL, and a short analysis time of down to 20 min, which makes it promising for clinical applications. The key parameters, including component concentrations, temperature, and incubation time, were optimized. The procedure was validated on 34 serum samples from healthy individuals and patients with cardiovascular diseases. To assess clinical applicability, a correlation analysis was performed between the electrochemical signal intensities and the concentrations obtained on the Mindray BS-800 and Getein-1100 analyzers. Statistical analysis showed strong linear correlations between the signal obtained by the developed procedure and the target protein concentrations in the samples: r = 0.96 with the Mindray BS-800 and r = 0.93 with the Getein-1100.